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The t-SNAREs syntaxin4 and SNAP23 but not v-SNARE VAMP2 are indispensable to tether GLUT4 vesicles at the plasma membrane in adipocyte
- Source :
- Biochemical and biophysical research communications. 391(3):1336-1341
- Publication Year :
- 2010
- Publisher :
- Elsevier, 2010.
-
Abstract
- SNARE proteins (VAMP2, syntaxin4, and SNAP23) have been thought to play a key role in GLUT4 trafficking by mediating the tethering, docking and subsequent fusion of GLUT4-containing vesicles with the plasma membrane. The precise functions of these proteins have remained elusive, however. We have now shown that depletion of the vesicle SNARE (v-SNARE) VAMP2 by RNA interference in 3T3-L1 adipocytes inhibited the fusion of GLUT4 vesicles with the plasma membrane but did not affect tethering of the vesicles to the membrane. In contrast, depletion of the target SNAREs (t-SNAREs) syntaxin4 or SNAP23 resulted in impairment of GLUT4 vesicle tethering to the plasma membrane. Our results indicate that the t-SNAREs syntaxin4 and SNAP23 are indispensable for the tethering of GLUT4 vesicles to the plasma membrane, whereas the v-SNARE VAMP2 is not required for this step but is essential for the subsequent fusion event.
- Subjects :
- Vesicle fusion
Vesicle-Associated Membrane Protein 2
Biophysics
Biology
Biochemistry
Membrane Fusion
Vesicle tethering
Exocytosis
Mice
3T3-L1 Cells
SNAP23
Adipocytes
Animals
Insulin
Qc-SNARE Proteins
Molecular Biology
Glucose Transporter Type 4
VAMP2
Tethering
Qa-SNARE Proteins
Vesicle
Cell Membrane
Cytoplasmic Vesicles
Cell Biology
Qb-SNARE Proteins
Cell biology
Protein Transport
Glucose
Porosome
RNA Interference
biological phenomena, cell phenomena, and immunity
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 391
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....153f62405ae3e7349e3312717dae6b33