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Connective tissue components in synovial fibroblast cultures exposed to interleukin 1 and prostaglandin E2
- Source :
- Virchows Archiv. B, Cell pathology including molecular pathology. 50(4)
- Publication Year :
- 1986
-
Abstract
- Long-term synovial fibroblast cultures were exposed to interleukin 1 (IL-1) or prostaglandin E2 (PGE2). The normally spindle-shaped fibroblasts changed to stellate-shaped cells, resembling the HLA-DR-positive, collagenase-producing cells which are normally seen only in primary cultures from enzyme-digested rheumatoid synovial tissue. However, the IL-1- or PGE2-induced fibroblasts were not HLA-DR-positive. This suggests that these cell populations represent originally different cell lines or that the expression of HLA-DR antigens is not induced by the agents used. For further characterization of these stellate cells, the location of fibronectin and type I collagen was studied by specific antibodies and the pericellular coat around fibroblasts was visualized by the erythrocyte exclusion method. Both IL-1 and PGE2 treatments destroyed the intercellular fibronectin network. Type I collagen was detected as intracellular granules. The stellate fibroblasts were usually full of these granules in contrast to intact fibroblasts in which the number of collagen fluorescence granules varied greatly. The pericellular coat known to be formed mainly by hyaluronic acid was similar around spindle and stellate-shaped fibroblasts. Rheumatoid arthritis-derived fibroblasts did not differ from their non-rheumatoid counterparts in any of the experiments. The effect of IL-1 and PGE2 on fibroblasts simulates the interaction between mononuclear cells and fibroblasts in synovial stroma and also potentially the interactions between different cell types in synovial lining.
- Subjects :
- Connective tissue
Fluorescent Antibody Technique
Dinoprostone
Arthritis, Rheumatoid
chemistry.chemical_compound
Hyaluronic acid
medicine
Humans
Fibroblast
Cells, Cultured
Connective Tissue Cells
biology
Prostaglandins E
Synovial Membrane
Histocompatibility Antigens Class II
HLA-DR Antigens
Cell biology
Fibronectin
medicine.anatomical_structure
Synovial Cell
chemistry
Connective Tissue
Immunology
Hepatic stellate cell
biology.protein
Collagen
Synovial membrane
Type I collagen
Interleukin-1
Subjects
Details
- ISSN :
- 03406075
- Volume :
- 50
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv. B, Cell pathology including molecular pathology
- Accession number :
- edsair.doi.dedup.....153b2bc0a30e8e62b3b84bf8938dd481