Back to Search
Start Over
MALDI-TOF mass spectrometry-based identification of group A Streptococcus isolated from areas of the 2011 scarlet fever outbreak in china
- Source :
- Infection, Genetics and Evolution. 14:320-326
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- There was a dramatic increase in scarlet fever cases in China from March to July 2011. Group A Streptococcus (GAS) is the only pathogen known to cause scarlet fever. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) coupled to Biotyper system was used for GAS identification in 2011. A local reference database (LRD) was constructed, evaluated and used to identify GAS isolates. The 75 GAS strains used to evaluate the LRD were all identified correctly. Of the 157 suspected β-hemolytic strains isolated from 298 throat swab samples, 127 (100%) and 120 (94.5%) of the isolates were identified as GAS by the MALDI-TOF MS system and the conventional bacitracin sensitivity test method, respectively. All 202 (100%) isolates were identified at the species level by searching the LRD, while 182 (90.1%) were identified by searching the original reference database (ORD). There were statistically significant differences with a high degree of credibility at species level (χ(2)=6.052, P
- Subjects :
- Microbiology (medical)
China
Databases, Factual
Scarlet Fever
Streptococcus pyogenes
Bacitracin
Biology
Mass spectrometry
medicine.disease_cause
Microbiology
Group A
Disease Outbreaks
Genetics
medicine
Cluster Analysis
Humans
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Streptococcus
Outbreak
medicine.disease
Matrix-assisted laser desorption/ionization
Infectious Diseases
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Scarlet fever
medicine.drug
Subjects
Details
- ISSN :
- 15671348
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Infection, Genetics and Evolution
- Accession number :
- edsair.doi.dedup.....153525e2e926ef6335036cb0c25c334b