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Analysis of Orientations of Collagen Fibers by Novel Fiber-Tracking Software

Authors :
J. Paul Robinson
Ching-Shoei Chiang
Jennie Sturgis
Christoph M. Hoffmann
Bartlomiej Rajwa
David L. Filmer
Jun Wu
Bo Yuan
Source :
Microscopy and Microanalysis. 9:574-580
Publication Year :
2003
Publisher :
Oxford University Press (OUP), 2003.

Abstract

Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to not only its composition but also its structure. This article integrates confocal microscopy imaging and image-processing techniques to analyze the microstructural properties of ECM. This report describes a two- and three-dimensional fiber middle-line tracing algorithm that may be used to quantify collagen fibril organization. We utilized computer simulation and statistical analysis to validate the developed algorithm. These algorithms were applied to confocal images of collagen gels made with reconstituted bovine collagen type I, to demonstrate the computation of orientations of individual fibers.

Details

ISSN :
14358115 and 14319276
Volume :
9
Database :
OpenAIRE
Journal :
Microscopy and Microanalysis
Accession number :
edsair.doi.dedup.....14f299f24a7056cc1d34a9d30311ff76
Full Text :
https://doi.org/10.1017/s1431927603030277