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Analysis of Orientations of Collagen Fibers by Novel Fiber-Tracking Software
- Source :
- Microscopy and Microanalysis. 9:574-580
- Publication Year :
- 2003
- Publisher :
- Oxford University Press (OUP), 2003.
-
Abstract
- Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to not only its composition but also its structure. This article integrates confocal microscopy imaging and image-processing techniques to analyze the microstructural properties of ECM. This report describes a two- and three-dimensional fiber middle-line tracing algorithm that may be used to quantify collagen fibril organization. We utilized computer simulation and statistical analysis to validate the developed algorithm. These algorithms were applied to confocal images of collagen gels made with reconstituted bovine collagen type I, to demonstrate the computation of orientations of individual fibers.
- Subjects :
- Materials science
Orientation (computer vision)
Confocal
Reproducibility of Results
Nanotechnology
Image processing
Tracing
Extracellular Matrix
law.invention
Extracellular matrix
Collagen fibril organization
Confocal microscopy
law
Animals
Cattle
Computer Simulation
Collagen
Fiber
Instrumentation
Algorithms
Software
Skin
Biomedical engineering
Subjects
Details
- ISSN :
- 14358115 and 14319276
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Microscopy and Microanalysis
- Accession number :
- edsair.doi.dedup.....14f299f24a7056cc1d34a9d30311ff76
- Full Text :
- https://doi.org/10.1017/s1431927603030277