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CXC chemokines and leukocyte chemotaxis in common carp (Cyprinus carpio L.)
- Source :
- Developmental and Comparative Immunology, 27(10), 875-888, Developmental and Comparative Immunology, 27, 875-888, Developmental and Comparative Immunology, 27, 10, pp. 875-888, Developmental and Comparative Immunology 27 (2003) 10
- Publication Year :
- 2003
-
Abstract
- CXC chemokines, structurally recognizable by the position of four conserved cysteine residues, are prominent mediators of chemotaxis. Here we report a novel carp CXC chemokine obtained through homology cloning and compare it with fish orthologues genes and with a second, recently elucidated, carp CXC chemokine. Phylogenetic analyses clearly show that neither CXC chemokine resembles any of the mammalian CXC chemokines in particular. However, basal expression is most prominent in immune organs like anterior kidney and spleen, suggesting involvement in the immune response. Furthermore we show that anterior kidney phagocyte-enriched leukocyte suspensions express both chemokines and that this expression is upregulated by brief (4 h) stimulation with PMA, but not lipopolysaccharide. Neutrophilic granulocyte-enriched leukocytes display chemotaxis to human recombinant CXCL8 (hrCXCL8; interleukin-8), confirming CXC chemokine mediated chemotaxis of neutrophilic granulocytes in teleost fish. Factors secreted from carp phagocytes are also capable of inducing chemotaxis and secretion of these factors into culture supernatants is upregulated by PMA. Finally we demonstrate involvement of both CXC chemokines as well as CXCR1 and CXCR2 in acute Argulus japonicus infection. Collectively the data presented implicate the involvement of CXC chemokines in chemotaxis of fish neutrophils in a fashion that shares characteristics with the mammalian situation. However, the CXC chemokines involved differ enough from those involved in neutrophil chemotaxis in mammals to warrant their own nomenclature. (C) 2003 Elsevier Science Ltd. All rights reserved.
- Subjects :
- Carps
Neutrophils
Molecular Sequence Data
Immunology
cloning
Sequence Homology
Neurophysiology
receptors
Celbiologie en Immunologie
Biology
Kidney
CXCR3
Animals
Humans
Amino Acid Sequence
CXC chemokine receptors
Cloning, Molecular
CXCL14
CX3CL1
Cells, Cultured
Phylogeny
fish
mechanisms
Base Sequence
Gene Expression Profiling
Chemotaxis
danger
respiratory system
immunity
Cell biology
Chemotaxis, Leukocyte
CXCL2
Gene Expression Regulation
Cell Biology and Immunology
WIAS
CXCL9
identification
Receptors, Chemokine
Organismal Animal Physiology
activation
Chemokines, CXC
Sequence Alignment
Leukocyte chemotaxis
trout oncorhynchus-mykiss
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 27
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Developmental and Comparative Immunology
- Accession number :
- edsair.doi.dedup.....14eef8a68fabb9372acb6bb02467115a