Back to Search
Start Over
Divergent Targets of Candida albicans Biofilm Regulator Bcr1 In Vitro and In Vivo
- Source :
- Eukaryotic Cell. 11:896-904
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- Candida albicans is a causative agent of oropharyngeal candidiasis (OPC), a biofilm-like infection of the oral mucosa. Biofilm formation depends upon the C. albicans transcription factor Bcr1, and previous studies indicate that Bcr1 is required for OPC in a mouse model of infection. Here we have used a nanoString gene expression measurement platform to elucidate the role of Bcr1 in OPC-related gene expression. We chose for assays a panel of 134 genes that represent a range of morphogenetic and cell cycle functions as well as environmental and stress response pathways. We assayed gene expression in whole infected tongue samples. The results sketch a portrait of C. albicans gene expression in which numerous stress response pathways are activated during OPC. This one set of experiments identifies 64 new genes with significantly altered RNA levels during OPC, thus increasing substantially the number of known genes in this expression class. The bcr1 Δ/Δ mutant had a much more limited gene expression defect during OPC infection than previously reported for in vitro growth conditions. Among major functional Bcr1 targets, we observed that ALS3 was Bcr1 dependent in vivo while HWP1 was not. We used null mutants and complemented strains to verify that Bcr1 and Hwp1 are required for OPC infection in this model. The role of Als3 is transient and mild, though significant. Our findings suggest that the versatility of C. albicans as a pathogen may reflect its ability to persist in the face of multiple stresses and underscore that transcriptional circuitry during infection may be distinct from that detailed during in vitro growth.
- Subjects :
- Male
Mutant
Biology
Microbiology
Fungal Proteins
Mice
Candidiasis, Oral
In vivo
Gene Expression Regulation, Fungal
Candida albicans
Gene expression
Animals
Humans
Molecular Biology
Gene
Transcription factor
Regulation of gene expression
Mice, Inbred BALB C
Mouth Mucosa
Articles
General Medicine
biology.organism_classification
Corpus albicans
stomatognathic diseases
Disease Models, Animal
Biofilms
Subjects
Details
- ISSN :
- 15359786 and 15359778
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Eukaryotic Cell
- Accession number :
- edsair.doi.dedup.....14e46aa99d9a34e0627e3d45a60b87d8