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Total protein concentration and diagnostic test results for gray wolf (Canis lupus) serum using Nobuto filter paper strips
- Source :
- Journal of wildlife diseases. 51(2)
- Publication Year :
- 2015
-
Abstract
- Nobuto filter paper strips are widely used for storing blood-serum samples, but the recovery of proteins from these strips following rehydration is unknown. Poor recovery of proteins could reduce the concentration of antibodies and antigens and reduce the sensitivity of diagnostic assays. We compared the protein concentration, and its association with test sensitivity, of eluted Nobuto strip samples with paired sera. We collected and froze serum from five gray wolves (Canis lupus) for 8 mo. When thawed, we used a spectrophotometer (absorbance 280 nm) to determine the serum protein concentration for paired sera and Nobuto eluates for each animal in 2-fold serial dilutions. Total protein concentration was similar for both sample storage methods (Nobuto eluates and control sera), except for the undiluted samples in which Nobuto eluates had higher total protein concentrations. Both sample storage methods appear to produce similar results using the SNAP® 4Dx® Test to detect antibodies against pathogens causing Lyme disease, anaplasmosis, and ehrlichiosis as well as antigen for canine heartworm disease.
- Subjects :
- Paper
Veterinary medicine
Serial dilution
Serum protein
Sensitivity and Specificity
Antibodies
Specimen Handling
medicine
Animals
Serologic Tests
Ecology, Evolution, Behavior and Systematics
Total protein
Blood Specimen Collection
Chromatography
Systemic lupus erythematosus
Wolves
Ecology
biology
Filter paper
Clinical Laboratory Techniques
Micropore Filters
Diagnostic test
Blood Proteins
biology.organism_classification
medicine.disease
Canis
biology.protein
Antibody
Dirofilaria
Subjects
Details
- ISSN :
- 19433700
- Volume :
- 51
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of wildlife diseases
- Accession number :
- edsair.doi.dedup.....14e2b7ba2d7945a511e718f23cfa20cf