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The presence of modified nucleotides is required for cloverleaf folding of a human mitochondrial tRNA
- Source :
- Nucleic Acids Research. 26:1636-1643
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- Direct sequencing of human mitochondrial tRNALysshows the absence of editing and the occurrence of six modified nucleotides (m1A9, m2G10, Psi27, Psi28 and hypermodified nucleotides at positions U34 and A37). This tRNA folds into the expected cloverleaf, as confirmed by structural probing with nucleases. The solution structure of the corresponding in vitro transcript unexpectedly does not fold into a cloverleaf but into an extended bulged hairpin. This non-canonical fold, established according to the reactivity to a large set of chemical and enzymatic probes, includes a 10 bp aminoacyl acceptor stem (the canonical 7 bp and 3 new pairs between residues 8-10 and 65-63), a 13 nt large loop and an anticodon-like domain. It is concluded that modified nucleotides have a predominant role in canonical folding of human mitochondrial tRNALys. Phylogenetic comparisons as well as structural probing of selected in vitro transcribed variants argue in favor of a major contribution of m1A9 in this process.
- Subjects :
- Models, Molecular
Transcription, Genetic
RNA, Mitochondrial
Cloning, Organism
Placenta
Mitochondrion
Biology
Methylation
Polymerase Chain Reaction
Pregnancy
Phylogenetics
Genetics
Humans
Nucleotide
Phylogeny
chemistry.chemical_classification
Genetic Variation
RNA
Mitochondria
Enzyme
chemistry
Biochemistry
RNA editing
Transfer RNA
Mutagenesis, Site-Directed
Nucleic Acid Conformation
RNA, Transfer, Lys
Female
RNA Editing
Research Article
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....14bca6d93fd7e58ca023b18ecd348b35