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The effect of anti-lymphocyte serum on subpopulations of blood and tissue leucocytes: possible supplementary mechanisms for suppression of rejection and the development of opportunistic infections
The effect of anti-lymphocyte serum on subpopulations of blood and tissue leucocytes: possible supplementary mechanisms for suppression of rejection and the development of opportunistic infections
- Source :
- Transplant International. 18:1366-1375
- Publication Year :
- 2005
- Publisher :
- Frontiers Media SA, 2005.
-
Abstract
- Xenogeneic anti-lymphocyte serum (ALS) remains a major reagent for immunosuppression in clinical practice, but mechanisms of action and risks of opportunistic infection have not been considered in the context of innate immunity and its role in immune responsiveness. Rabbit anti rat ALS was administered intraperitoneally. Blood was taken for flow cytometry to establish absolute counts of leucocyte subsets. Tissues were harvested for immunohistology to evaluate interstitial dendritic cells and tissue macrophages. At day 2 of ALS therapy, T cells are completely depleted, as anticipated. B cells are undiminished and form approximately 90% of blood leucocytes. Monocytes and natural killer (NK) cells are substantially (approximately 80%), but not completely, depleted, and there is a trend for diminished numbers of putative dendritic cells. Neither interstitial dendritic cells nor tissue macrophages in heart are affected. The results at day 7 were very similar to day 2. Substantial depletion of blood monocytes and NK cells might attenuate the innate immune system, and represent a possible supplementary mechanism (in addition to T cell depletion) for suppression of rejection. It might be of particular importance in reducing defences against infections. Monitoring these parameters could be of clinical value.
- Subjects :
- Male
Time Factors
Opportunistic infection
Lymphocyte
medicine.medical_treatment
Cell Separation
Opportunistic Infections
Monocytes
Flow cytometry
Immune system
Species Specificity
Leukocytes
Animals
Medicine
Antigen-presenting cell
Antilymphocyte Serum
Transplantation
Innate immune system
medicine.diagnostic_test
business.industry
Macrophages
Antibodies, Monoclonal
Immunosuppression
Dendritic Cells
Dendritic cell
Flow Cytometry
medicine.disease
Immunohistochemistry
Blood Cell Count
Rats
Killer Cells, Natural
Treatment Outcome
medicine.anatomical_structure
Microscopy, Fluorescence
Rats, Inbred Lew
Immunology
Heart Transplantation
Rabbits
business
Immunosuppressive Agents
Subjects
Details
- ISSN :
- 14322277 and 09340874
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Transplant International
- Accession number :
- edsair.doi.dedup.....14a924ba079688d00a712cf6b64e2660
- Full Text :
- https://doi.org/10.1111/j.1432-2277.2005.00211.x