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Estrogen prevents intestinal inflammation after trauma-hemorrhage via downregulation of angiotensin II and angiotensin II subtype I receptor
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 295:G1131-G1137
- Publication Year :
- 2008
- Publisher :
- American Physiological Society, 2008.
-
Abstract
- Although angiotensin II (Ang II) plays a key role in development of organ ischemia-reperfusion injury, it remains unclear whether it is involved in development of intestinal injury following trauma-hemorrhage (T-H). Studies have shown that 17β-estradiol (E2) administration following T-H improves small intestinal blood flow; however, it is unclear whether Ang II plays a role in this E2-mediated salutary effect. Male Sprague-Dawley rats underwent laparotomy and hemorrhagic shock (removal of 60% total blood volume, fluid resuscitation after 90 min). At onset of resuscitation, rats were treated with vehicle, E2, or E2 and estrogen receptor antagonist ICI 182,780 (ICI). A separate group of rats was treated with Ang II subtype I receptor (AT1R) antagonist losartan. At 24 h after T-H, plasma Ang II, IL-6, TNF-α, intercellular adhesion molecule (ICAM)-1, cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-3 levels, myeloperoxidase (MPO) activity, and AT1R expression were determined. T-H significantly increased plasma and intestinal Ang II, IL-6, TNF-α levels, intestinal ICAM-1, CINC-1, CINC-3 levels, MPO activity, and AT1R protein compared with shams. E2 treatment following T-H attenuated increased intestinal MPO activity, Ang II level, and AT1R protein expression. ICI administration abolished the salutary effects of E2. In contrast, losartan administration attenuated increased MPO activity without affecting Ang II and AT1R levels. Thus Ang II plays a role in producing small intestine inflammation following T-H, and the salutary effects of E2 on intestinal inflammation are mediated in part by Ang II and AT1R downregulation.
- Subjects :
- Male
medicine.medical_specialty
Angiotensin receptor
Physiology
medicine.drug_class
medicine.medical_treatment
Hormones and Signaling
Down-Regulation
Hemorrhage
Inflammation
Receptor, Angiotensin, Type 1
Rats, Sprague-Dawley
Downregulation and upregulation
Physiology (medical)
Internal medicine
Renin–angiotensin system
Animals
Medicine
Receptor
Fulvestrant
Peroxidase
Estradiol
Hepatology
business.industry
Angiotensin II
Gastroenterology
Rats
Endocrinology
Cytokine
Estrogen
Cytokines
medicine.symptom
business
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 295
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....14a74dc02a9768ffb15efd14c6b64573