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Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion
- Source :
- Turaihi, A H, van Poelgeest, E M, van Hinsbergh, V W M, Serné, E H, Smulders, Y M & Eringa, E C 2017, ' Combined intravital microscopy and contrast-enhanced ultrasonography of the mouse hindlimb to study insulin-induced vasodilation and muscle perfusion ', Journal of Visualized Experiments, vol. 2017, no. 121, e54912 . https://doi.org/10.3791/54912, Journal of Visualized Experiments, 2017(121):e54912. MYJoVE Corporation, Journal of Visualized Experiments : JoVE
- Publication Year :
- 2017
- Publisher :
- MyJove Corporation, 2017.
-
Abstract
- It has been demonstrated that insulin’s vascular actions contribute to regulation of insulin sensitivity. Insulin’s effects on muscle perfusion regulate postprandial delivery of nutrients and hormones to insulin-sensitive tissues. We here describe a technique for combining intravital microscopy (IVM) and contrast-enhanced ultrasonography (CEUS) of the adductor compartment of the mouse hindlimb to simultaneously visualize muscle resistance arteries and perfusion of the microcirculation in vivo. Simultaneously assessing insulin’s effect at multiple levels of the vascular tree is important to study relationships between insulin’s multiple vasoactive effects and muscle perfusion. Experiments in this study were performed in mice. First, the tail vein cannula is inserted for the infusion of anesthesia, vasoactive compounds and ultrasound contrast agent (lipid-encapsulated microbubbles). Second, a small incision is made in the groin area to expose the arterial tree of the adductor muscle compartment. The ultrasound probe is then positioned at the contralateral upper hindlimb to view the muscles in cross-section. To assess baseline parameters, the arterial diameter is assessed and microbubbles are subsequently infused at a constant rate to estimate muscle blood flow and microvascular blood volume (MBV). When applied before and during a hyperinsulinemic-euglycemic clamp, combined IVM and CEUS allow assessment of insulin-induced changes of arterial diameter, microvascular muscle perfusion and whole-body insulin sensitivity. Moreover, the temporal relationship between responses of the microcirculation and the resistance arteries to insulin can be quantified. It is also possible to follow-up the mice longitudinally in time, making it a valuable tool to study changes in vascular and whole-body insulin sensitivity.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Intravital Microscopy
endothelium
General Chemical Engineering
medicine.medical_treatment
Contrast Media
Vasodilation
Hindlimb
General Biochemistry, Genetics and Molecular Biology
Microcirculation
Mice
03 medical and health sciences
medicine
Animals
Hypoglycemic Agents
Insulin
insulin sensitivity
Muscle, Skeletal
Ultrasonography
contrast-enhanced ultrasonography
Microbubbles
General Immunology and Microbiology
business.industry
General Neuroscience
microsurgery
Glucose clamp technique
Arterial tree
Femoral Artery
Issue 121
030104 developmental biology
Glucose Clamp Technique
Medicine
vascular responsiveness
business
Perfusion
Intravital microscopy
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....14a604c6411ec0a5195f2bc13a9f33cb