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Characterization of the gene encoding glycoprotein C of duck enteritis virus
- Source :
- Virus Genes. 37:328-332
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- A total of 2,718 bp of DNA fragment was amplified from the C-KCE strain of duck enteritis virus (DEV) genome using thermal asymmetric interlaced PCR. This newly identified viral DNA fragment contained two non-overlapping open reading frames (ORFs) oriented from the 5' to 3' direction. The first ORF was comprised of 43.5% G + C and contained the full-length genomic sequence of the UL44 gene (1,296 bp) encoding 431 amino acid residues of DEV glycoprotein C (gC). The second ORF encoded a partial peptide of the UL43 gene. The sequences of DNA and deduced amino acids of the DEV gC gene shared high homology with other members of known herpesviruses, supporting the classification of DEV. Phylogenetic analysis of the DEV gC gene revealed that the gC gene had a close evolutionary relationship with the subfamily of Alphaherpesvirinae.
- Subjects :
- animal structures
Subfamily
Molecular Sequence Data
Genome
Evolution, Molecular
Chromosome Walking
Open Reading Frames
chemistry.chemical_compound
Viral Envelope Proteins
Virology
Alphaherpesvirinae
Genetics
Animals
Amino Acid Sequence
Cloning, Molecular
ORFS
Molecular Biology
Gene
Cells, Cultured
Herpesviridae
Phylogeny
Base Composition
Base Sequence
biology
Phylogenetic tree
General Medicine
biology.organism_classification
Molecular biology
Open reading frame
Ducks
chemistry
embryonic structures
Chickens
DNA
Subjects
Details
- ISSN :
- 1572994X and 09208569
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Virus Genes
- Accession number :
- edsair.doi.dedup.....14a43f3e14a9a5b044d2120e9235584c