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β-Catenin Is Not Necessary for Maintenance or Repair of the Bronchiolar Epithelium
- Source :
- American Journal of Respiratory Cell and Molecular Biology. 41:535-543
- Publication Year :
- 2009
- Publisher :
- American Thoracic Society, 2009.
-
Abstract
- Signaling by Wnt/beta-catenin regulates self-renewal of tissue stem cells in the gut and, when activated in the embryonic bronchiolar epithelium, leads to stem cell expansion. We have used transgenic and cell type-specific knockout strategies to determine roles for beta-catenin-regulated gene expression in normal maintenance and repair of the bronchiolar epithelium. Analysis of TOPGal transgene activity detected beta-catenin signaling in the steady-state and repairing bronchiolar epithelium. However, the broad distribution and phenotype of signaling cells precluded establishment of a clear role for beta-catenin in the normal or repairing state. Necessity of beta-catenin signaling was tested through Cre-mediated deletion of Catnb exons 2-6 in airway epithelial cells. Functional knockout of beta-catenin had no impact on expression of Clara cell differentiation markers, mitotic index, or sensitivity of these cells to the Clara cell-specific toxicant, naphthalene. Repair of the naphthalene-injured airway proceeded with establishment of focal regions of beta-catenin-null epithelium. The size of regenerative epithelial units, mitotic index, and restoration of the ciliated cell population did not vary between wild-type and genetically modified mice. Thus, beta-catenin was not necessary for maintenance or efficient repair of the bronchiolar epithelium.
- Subjects :
- Pulmonary and Respiratory Medicine
Time Factors
Cellular differentiation
Clinical Biochemistry
Clara cell differentiation
Population
Mice, Transgenic
Respiratory Mucosa
Naphthalenes
Biology
Gene Knockout Techniques
Mice
Mitotic Index
medicine
Animals
Regeneration
Uteroglobin
RNA, Messenger
Progenitor cell
Promoter Regions, Genetic
education
Bronchioles
Molecular Biology
beta Catenin
Cell Proliferation
education.field_of_study
Integrases
Stem Cells
Wnt signaling pathway
Gene Expression Regulation, Developmental
Cell Differentiation
Articles
Cell Biology
respiratory system
Embryonic stem cell
Epithelium
Rats
Cell biology
Mice, Inbred C57BL
Phenotype
medicine.anatomical_structure
Lac Operon
Immunology
Stem cell
TCF Transcription Factors
Transcription Factor 7-Like 2 Protein
Signal Transduction
Subjects
Details
- ISSN :
- 15354989 and 10441549
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- American Journal of Respiratory Cell and Molecular Biology
- Accession number :
- edsair.doi.dedup.....149acbbae46634d70caf31f53ef53202