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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
- Source :
- Journal of visualized experiments : JoVE. (160)
- Publication Year :
- 2020
-
Abstract
- Studying isolated cells from mucosa-associated lymphoid tissues (MALT) allows understanding of immune cells response in pathologies involving mucosal immunity, because they can model host-pathogen interactions in the tissue. While isolated cells derived from tissues were the first cell culture model, their use has been neglected because tissue can be hard to obtain. In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells (TMCs) from healthy human tonsils to study innate immune responses upon activation, mimicking viral infection in mucosal tissues. Isolation of TMCs from the tonsils is quick, because the tonsils barely have any epithelium and yield up to billions of all major immune cell types. This method allows detection of cytokine production using several techniques, including immunoassays, qPCR, microscopy, flow cytometry, etc., similar to the use of peripheral mononuclear cells (PBMCs) from blood. Furthermore, TMCs show a higher sensitivity to drug testing than PBMCs, which needs to be considered for future toxicity assays. Thus, ex vivo TMCs cultures are an easy and accessible mucosal model.
- Subjects :
- Innate immune system
medicine.diagnostic_test
General Immunology and Microbiology
Lymphoid Tissue
General Chemical Engineering
General Neuroscience
Palatine Tonsil
Cell Separation
Biology
Flow Cytometry
Peripheral blood mononuclear cell
General Biochemistry, Genetics and Molecular Biology
Immunity, Innate
Flow cytometry
Immune system
medicine.anatomical_structure
Lymphatic system
Tonsil
Immunology
medicine
Leukocytes, Mononuclear
Humans
Mucosa-associated lymphoid tissue
Immunity, Mucosal
Ex vivo
Subjects
Details
- ISSN :
- 1940087X
- Issue :
- 160
- Database :
- OpenAIRE
- Journal :
- Journal of visualized experiments : JoVE
- Accession number :
- edsair.doi.dedup.....1498e891c5b5cca53afc5c2d9cc9d316