Simultaneous estimation of rates of DNA damage induced by three important chemotherapy drugs by a novel electrochemical biosensor assisted by chemometric multivariate calibration methods

In this work, a novel electrochemical biosensor assisted by multivariate calibration methods was developed for simultaneous estimation of rates of DNA damage induced by doxorubicin (DX), daunorubicin (DR) and idarubicin (ID), and also to simultaneous determination of the drugs. A glassy carbon electrode was efficiently modified and used as the biosensing platform. Binding and interactions of DX, DR and ID with DNA were modeled by molecular docking methods, and theoretical information was completed by experimental results. The methylene blue was able to intercalate within the DNA structure and by incubation of the biosensor with DX or DR or ID, the methylene blue was replaced by drug and therefore, the voltammetric signal of the biosensor was changed due to the exposed DNA and repelling the electrochemical probe molecules carrying negative charge. The DNA damage induced by each drug was individually monitored by differential pulse voltammetry and then, rates of DNA damage were calibrated and validated by mixture design and multivariate calibration methods. The developed multivariate calibration model constructed based on vectorization of the data was able to simultaneous detection of the rates of DNA damage induced by all the three drugs. The change in the biosensor response in the presence of the drugs was also modeled by multivariate calibration methods to simultaneous determination of the drugs.