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LMNA-mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy
- Source :
- Aging and Disease
- Publication Year :
- 2019
- Publisher :
- Aging and Disease, 2019.
-
Abstract
- Premature aging syndromes are rare genetic disorders mimicking clinical and molecular features of aging. Products of the LMNA gene, primarily lamin A and C, are major components of the nuclear lamina. A recently identified group of premature aging syndromes was related to mutations of the LMNA gene. Although LMNA disorders have been identified in premature aging syndromes, affect specifically the skeletal muscles, cardiac muscles, and lipodystrophy, understanding the pathogenic mechanisms still need to be elucidated. Here, to establish a rabbit knockout (KO) model of premature aging syndromes, we performed precise LMNA targeting in rabbits via co-injection of Cas9/sgRNA mRNA into zygotes. The LMNA-KO rabbits exhibited reduced locomotion activity with abnormal stiff walking posture and a shortened stature, all of them died within 22 days. In addition, cardiomyopathy, muscular dystrophy, bone and joint abnormalities, as well as lipodystrophy were observed in LMNA-KO rabbits. In conclusion, the novel rabbit LMNA-KO model, displayed typical features of histopathological defects that are observed in premature aging syndromes, and may be utilized as a valuable resource for understanding the pathophysiological mechanisms of premature aging syndromes and elucidating mysteries of the normal process of aging in humans.
- Subjects :
- 0301 basic medicine
Premature aging
congenital, hereditary, and neonatal diseases and abnormalities
Pathology
medicine.medical_specialty
rabbit
Cardiomyopathy
LMNA
Orginal Article
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
medicine
Muscular dystrophy
CRISPR/Cas9
integumentary system
business.industry
Dilated cardiomyopathy
Cell Biology
premature aging syndrome
medicine.disease
Pathophysiology
030104 developmental biology
Neurology (clinical)
Geriatrics and Gerontology
Lipodystrophy
business
030217 neurology & neurosurgery
Lamin
Subjects
Details
- ISSN :
- 21525250
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Aging and disease
- Accession number :
- edsair.doi.dedup.....1490d326cffed3551b3f246dcd17777c