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Pantoprazole, a proton‐pump inhibitor, impairs human sperm motility and capacitation in vitro
- Source :
- Andrology, Andrology, Wiley, 2020, 8 (6), pp.1795-1804. ⟨10.1111/andr.12855⟩, Andrology (2020)
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Background The effects of PPIs on human sperm fertilizing capacity were poorly investigated although these drugs are widely over-used. Two publications retrospectively studied relationships between any PPI intake and sperm parameters from patients consulting at infertility clinics, but the conclusions of these reports were contradictory. Only two reports investigated the effects of lansoprazole and omeprazole on sperm motility and found lansoprazole to be deleterious and omeprazole to be neutral for sperm motility. The inconsistency of the PPI effect in the previous reports emphasizes the need for more basic research on human spermatozoa, taking into account the hypothesis that the different PPI drugs may have different effects on sperm physiology. Objectives Do PPIs, which are among the most widely sold drug in the word, impact negatively human sperm capacitation and sperm motility? Materials and methods The effects of PPIs on human sperm maturation and motility were analyzed by CASA, flow cytometry, and Western blot. Results We tested the impact of 6 different PPIs on human sperm motility and capacitation. We showed that pantoprazole, but not the other PPIs, decreased sperm progressive motility and capacitation-induced sperm hyperactivation. We therefore investigated further the effects of pantoprazole on sperm capacitation, and we observed that it had a significant deleterious effect on the capacitation-induced hyperpolarization of the membrane potential and capacitation-associated protein phosphorylation. Discussion and conclusion Our results indicate that exposure to pantoprazole has an adverse effect on the physiological competence of human spermatozoa. As the capacitation process takes place within the female tract, our results suggest that PPIs intake by the female partner may impair in vivo sperm maturation and possibly fertilization. Moreover, the absence of adverse effect by PPIs on mouse sperm emphasizes the need to develop reprotox assays using human material to better assess the effects of medication intake on sperm physiology.
- Subjects :
- Adult
Male
endocrine system
Urology
Endocrinology, Diabetes and Metabolism
[SDV]Life Sciences [q-bio]
Lansoprazole
Motility
2-Pyridinylmethylsulfinylbenzimidazoles
Membrane Potentials
Andrology
03 medical and health sciences
Young Adult
0302 clinical medicine
Endocrinology
Human fertilization
Capacitation
medicine
Humans
ddc:576.5
Phosphorylation
Pantoprazole
reproductive and urinary physiology
Sperm motility
ComputingMilieux_MISCELLANEOUS
Retrospective Studies
030219 obstetrics & reproductive medicine
Hyperactivation
urogenital system
business.industry
Proton Pump Inhibitors
Middle Aged
Sperm
Spermatozoa
3. Good health
Semen Analysis
Sperm Maturation
Reproductive Medicine
Fertilization
Rabeprazole
Sperm Motility
business
Sperm Capacitation
Omeprazole
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20472927 and 20472919
- Database :
- OpenAIRE
- Journal :
- Andrology, Andrology, Wiley, 2020, 8 (6), pp.1795-1804. ⟨10.1111/andr.12855⟩, Andrology (2020)
- Accession number :
- edsair.doi.dedup.....148929a1686da2538387c11ec219b54c