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Heparanase Localization during Palatogenesis in Mice
- Source :
- BioMed Research International, BioMed Research International, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Publishing Corporation, 2013.
-
Abstract
- Palatogenesis is directed by epithelial-mesenchymal interactions and results partly from remodeling of the extracellular matrix (ECM) of the palatal shelves. Here, we assessed heparanase distribution in developing mouse palates. No heparanase was observed in the vertically oriented palatal shelves in early stages of palate formation. As palate formation progressed, the palatal shelves were reorganized and arranged horizontally above the tongue, and heparanase localized to the epithelial cells of these shelves. When the palatal bilateral shelves first made contact, the heparanase localized to epithelial cells at the tips of shelves. Later in fusing palatal shelves, the cells of the medial epithelial seam (MES) were labeled with intense heparanase signal. In contrast, the basement membrane heparan sulfate (HS) was scarcely observed in the palatal shelves in contact. Moreover, perlecan labeling was sparse in the basement membrane of the MES, on which laminin and type IV collagen were observed. Moreover, we assessed the distribution of matrix metalloproteinase- (MMP-) 9, MMP-2, and MMP-3 in developing mouse palates and these MMPs were observed in the MES. Our findings indicated that heparanase was important for palate formation because it mediated degradation of the ECM of palatal shelves. Heparanase may, in concert with other proteases, participate in the regression of the MES.
- Subjects :
- Collagen Type IV
Male
Time Factors
Article Subject
lcsh:Medicine
Perlecan
Matrix metalloproteinase
General Biochemistry, Genetics and Molecular Biology
Basement Membrane
Gene Expression Regulation, Enzymologic
Extracellular matrix
chemistry.chemical_compound
Type IV collagen
Mice
Laminin
medicine
Animals
Heparanase
Glucuronidase
Basement membrane
General Immunology and Microbiology
biology
Chemistry
Palate
lcsh:R
Gene Expression Regulation, Developmental
General Medicine
Heparan sulfate
Anatomy
Cell biology
Extracellular Matrix
Mice, Inbred C57BL
medicine.anatomical_structure
Microscopy, Fluorescence
biology.protein
Female
Heparitin Sulfate
Heparan Sulfate Proteoglycans
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....14724b27c1e62eb1bd214b1473234b96