Back to Search Start Over

Heparanase Localization during Palatogenesis in Mice

Authors :
Nagato Natsume
Toshio Sugahara
Azumi Hirata
Yoshinori Otsuki
Kazufumi Takano
Hiroaki Nakamura
Kentaro Katayama
Yuichi Koga
Takehito Tsuji
Source :
BioMed Research International, BioMed Research International, Vol 2013 (2013)
Publication Year :
2013
Publisher :
Hindawi Publishing Corporation, 2013.

Abstract

Palatogenesis is directed by epithelial-mesenchymal interactions and results partly from remodeling of the extracellular matrix (ECM) of the palatal shelves. Here, we assessed heparanase distribution in developing mouse palates. No heparanase was observed in the vertically oriented palatal shelves in early stages of palate formation. As palate formation progressed, the palatal shelves were reorganized and arranged horizontally above the tongue, and heparanase localized to the epithelial cells of these shelves. When the palatal bilateral shelves first made contact, the heparanase localized to epithelial cells at the tips of shelves. Later in fusing palatal shelves, the cells of the medial epithelial seam (MES) were labeled with intense heparanase signal. In contrast, the basement membrane heparan sulfate (HS) was scarcely observed in the palatal shelves in contact. Moreover, perlecan labeling was sparse in the basement membrane of the MES, on which laminin and type IV collagen were observed. Moreover, we assessed the distribution of matrix metalloproteinase- (MMP-) 9, MMP-2, and MMP-3 in developing mouse palates and these MMPs were observed in the MES. Our findings indicated that heparanase was important for palate formation because it mediated degradation of the ECM of palatal shelves. Heparanase may, in concert with other proteases, participate in the regression of the MES.

Details

Language :
English
ISSN :
23146141 and 23146133
Volume :
2013
Database :
OpenAIRE
Journal :
BioMed Research International
Accession number :
edsair.doi.dedup.....14724b27c1e62eb1bd214b1473234b96