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An X-ray Scattering Study of Bromegrass Mosaic Virus
- Source :
- Biophysical Journal. 3(2):175-182
- Publication Year :
- 1963
- Publisher :
- Elsevier BV, 1963.
-
Abstract
- X-ray scattering data and electron microscope observations are presented for bromegrass mosaic virus. Its radial density distribution is obtained from the Fourier transform of the amplitudes of the scattered x-rays. The results indicate that the virus is 260 A in diameter, it has an almost empty central cavity which is about 80 A in diameter, and the regions occupied by RNA and protein are approximately equal in average density. Electron micrographs of negatively stained preparations also give an outside diameter of 260 A and indicate that there is a central region about 90 A in diameter into which uranyl acetate can penetrate. Positively stained preparations indicate that the nucleic acid is concentrated in a shell-shaped region which is in turn surrounded by a shell of protein. In order for the RNA and protein regions to have the same average electron density the RNA must have a hydration of 1.29 gm of water per gm of RNA and the protein must have a hydration of 0.24 gm of water per gm of protein.
- Subjects :
- Electron density
Bromus
Analytical chemistry
Biophysics
Uranyl acetate
Biology
Negative Staining
Plant Viruses
law.invention
chemistry.chemical_compound
law
Plant virus
Fourier Analysis
Mosaic virus
X-Rays
Proteins
RNA
Articles
Bromovirus
Negative stain
Crystallography
chemistry
Viruses
Nucleic acid
RNA, Viral
Electron microscope
Subjects
Details
- ISSN :
- 00063495
- Volume :
- 3
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....14708552c16a63f04d4efa8fa0b17b03
- Full Text :
- https://doi.org/10.1016/s0006-3495(63)86813-7