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Spermatogenesis-related ring finger gene ZNF230 promoter: identification and functional analysis
- Source :
- Xu, W, Zhang, S, Qiu, W, He, G, Liu, Y, Sun, Y, Ma, Y, Dong, J & Zhang, W 2009, ' Spermatogenesis-related ring finger gene ZNF230 promoter: identification and functional analysis ', Molecular Biology Reports, vol. 36, no. 5, pp. 1187-1193 . https://doi.org/10.1007/s11033-008-9296-2
- Publication Year :
- 2008
-
Abstract
- Udgivelsesdato: 2009-May The ZNF230 gene is a recently cloned gene which is transcribed only in fertile male testes and may be related to human spermatogenesis. To characterize the multiple stage-specific transcription elements necessary for ZNF230 expression, we cloned ZNF230 promoter and constructed chimeric luciferase reporter Plasmids. Overexpression and site-directed mutation test were used to characterize the cis-element. The results showed ZNF230 gene promoter to be GC rich and not contain a TATA box. Deletion analysis of the 5'-flanking region of ZNF230 in HEK293 cells indicated that the sequence encompassing from nt -131 to +152 has a basal transcriptional activity. Site-directed mutation test and mithramycin A treatment demonstrated that the ZNF230 promoter contained a functional Sp1 site. Overexpression of the Sox5 protein activated the promoter activity. A 312-bp fragment surrounding the transcription start site exhibits a characteristic CpG island which overlaps with the promoter region. We also provided evidence that both the human and mouse znf230 promoter consist of Sp1 binding site and GC-rich sequences, suggesting Sp1 is required for the transcription of human and mouse ZNF230 genes. In conclusion, these findings suggest that ZNF230 is tightly controlled at transcriptional level and a common mechanism controls the basal transcription of ZNF230 gene.
- Subjects :
- Male
Transcription, Genetic
Sp1 Transcription Factor
TATA box
Response element
5' flanking region
Molecular Sequence Data
CAAT box
Biology
Mice
Epigenetics of physical exercise
Sequence Homology, Nucleic Acid
Genetics
Animals
Humans
Promoter Regions, Genetic
Spermatogenesis
Molecular Biology
Sequence Deletion
Sp1 transcription factor
Binding Sites
General transcription factor
Base Sequence
Promoter
General Medicine
Sequence Analysis, DNA
Molecular biology
Spermatozoa
DNA-Binding Proteins
Gene Expression Regulation
Organ Specificity
CpG Islands
SOXD Transcription Factors
Sequence Alignment
Transcription Factors
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 36
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....146c0efc669252683acf7bd40cf605fc