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Effects of different components of the extracellular matrix on endothelialization
- Source :
- Krueger-Genge, A.; Jung, F.; Fuhrmann, R.; Franke, R.-P.: Effects of different components of the extracellular matrix on endothelialization. In: Clinical Hemorheology and Microcirculation. Vol. 64 (2016) 4, 867-874. (DOI: 10.3233/CH-168051)
- Publication Year :
- 2016
- Publisher :
- IOS Press, 2016.
-
Abstract
- The endothelialization of cardiovascular prostheses is known to improve their haemocompatibility. As such body-foreign materials often do not endothelialize spontaneously. A lot of in vitro studies are ongoing how endothelialization of biomaterials can be improved. In this study the influence of different components of a tissue-typical extracellular matrix (ECM) like laminin, fibronectin or gelatin on the formation of an endothelial cell monolayer and on the shear resistance of adherent cells on these substrates was studied.The study revealed that the density of human venous endothelial cells (HUVEC) monolayers differed markedly between cells grown on a natural ECM and cells grown on singularized components of an ECM (p < 0.001). Only HUVEC grown on laminin showed similar densities and a stress fiber pattern comparable to HUVEC grown on the ECM. HUVEC grown on gelatin- or fibronectin-coated coverslips were less firmly attached to the substrate; frequently individual HUVEC and even groups of cells detached.Concluding it seems that coating of implants with laminin supports the formation of shear resistant endothelial cell (EC) monolayer - superior to other ECM components.
- Subjects :
- 0301 basic medicine
Stress fiber
food.ingredient
Physiology
02 engineering and technology
Gelatin
Extracellular matrix
03 medical and health sciences
food
Laminin
Physiology (medical)
Monolayer
Humans
Cells, Cultured
biology
Chemistry
Endothelial Cells
Hematology
021001 nanoscience & nanotechnology
In vitro
Extracellular Matrix
Endothelial stem cell
Fibronectin
030104 developmental biology
Biophysics
biology.protein
Endothelium, Vascular
0210 nano-technology
Cardiology and Cardiovascular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 13860291
- Database :
- OpenAIRE
- Journal :
- Krueger-Genge, A.; Jung, F.; Fuhrmann, R.; Franke, R.-P.: Effects of different components of the extracellular matrix on endothelialization. In: Clinical Hemorheology and Microcirculation. Vol. 64 (2016) 4, 867-874. (DOI: 10.3233/CH-168051)
- Accession number :
- edsair.doi.dedup.....1469ece3ce4f0dbd5d2ecf9a4b72ed54