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Fibrinolytic components in nasal mucosa and nasal secretion
- Source :
- Histochemistry and Cell Biology
- Publication Year :
- 1998
- Publisher :
- Springer-Verlag, 1998.
-
Abstract
- We evaluated a possible role for fibrinolytic components in nasal secretion by tissue localization with immunohistochemical techniques and by measuring their antigen concentrations in nasal discharge by means of ELISA and fibrin autography. Nasal mucosa was obtained surgically from the inferior turbinate. Urokinase-type plasminogen activator (u-PA) specific staining was observed in pseudostratified ciliated epithelium and was predominant in mucous cells of the seromucinous gland, while serous cells were almost devoid of stain. The pattern of staining of plasminogen activator inhibitor-2 was similar to that of u-PA. In contrast, plasminogen activator inhibitor-1 (PAI-1) immunoreactive material was localized exclusively in serous cells of seromucinous glands. Positive staining for tissue-type plasminogen activator (t-PA) was observed in endothelial cells and basal cells, which differentiate into either ciliated or goblet cells. Nasal secretions were partially fractionated by immunospecific antibody-immobilized Sepharose. Subsequent fibrin autography patterns indicated the presence of u-PA, PAI-1, and t-PA. After methacholine provocation, the level of t-PA increased transiently but decreased rapidly with subsequent challenges. These differential stainings of fibrinolytic components and the existence of PAs and PAI-1 in the nasal discharge suggest that the fibrinolytic system may play a role in the movement and fluidity of nasal secretion.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Histology
medicine.medical_treatment
Mucous membrane of nose
Enzyme-Linked Immunosorbent Assay
Fibrin
Antigen
Fibrinolysis
Plasminogen Activator Inhibitor 1
medicine
Humans
Molecular Biology
Original Paper
biology
Methacholine
Chemistry
Mucous Cell
Cell Biology
Immunohistochemistry
Urokinase-Type Plasminogen Activator
Staining
Medical Laboratory Technology
Serous fluid
Nasal Mucosa
Nasal Discharge
biology.protein
Autoradiography
Female
Plasminogen Activator
Plasminogen activator
Subjects
Details
- Language :
- English
- ISSN :
- 1432119X and 09486143
- Volume :
- 110
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Histochemistry and Cell Biology
- Accession number :
- edsair.doi.dedup.....1467ba071ad4c4dd1454b605ffec217e