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Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias

Authors :
Prapin Wilairat
Uday Younis Hussein Abdullah
Noraesah Mahmud
Hishamshah Ibrahim
Bin Alwi Zilfalil
Haitham M. Jassim
Mohd Nur Fakhruzzaman Noorizhab
Suthat Fucharoen
Syafiq Asnawi Zainal Abidin
Teh L. Kek
Iekhsan Othman
Mohamad Zaki Salleh
Source :
Hemoglobin. 43(3)
Publication Year :
2019

Abstract

This is the first report of quadrupole time-of-flight (Q-TOF) mass spectrometric identification of the hemoglobin (Hb) subunits, α, β, δ and γ peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the cation exchange chromatography of Hb. The objectives were to identify the unknown high performance liquid chromatography (HPLC) peaks in healthy subjects and in patients with β-thalassemia (β-thal). The results demonstrate the existence of pools of free globin chains in red blood cells (RBCs). The α-, β-, δ- and γ-globin peptides were identified in the unknown HPLC peaks. The quantification and role of the free globin pool in patients with β-thal requires further investigation. Identification of all types of Hb subunits in the retention time (RT) before 1 min. suggests that altered Hbs is the nature of these fast-eluting peaks. Relevancy of thalassemias to the protein-aggregation disorders will require review of the role of free globin in the pathology of the disease.

Details

ISSN :
1532432X
Volume :
43
Issue :
3
Database :
OpenAIRE
Journal :
Hemoglobin
Accession number :
edsair.doi.dedup.....14640f9742778038b34f386726c432da