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Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias
- Source :
- Hemoglobin. 43(3)
- Publication Year :
- 2019
-
Abstract
- This is the first report of quadrupole time-of-flight (Q-TOF) mass spectrometric identification of the hemoglobin (Hb) subunits, α, β, δ and γ peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the cation exchange chromatography of Hb. The objectives were to identify the unknown high performance liquid chromatography (HPLC) peaks in healthy subjects and in patients with β-thalassemia (β-thal). The results demonstrate the existence of pools of free globin chains in red blood cells (RBCs). The α-, β-, δ- and γ-globin peptides were identified in the unknown HPLC peaks. The quantification and role of the free globin pool in patients with β-thal requires further investigation. Identification of all types of Hb subunits in the retention time (RT) before 1 min. suggests that altered Hbs is the nature of these fast-eluting peaks. Relevancy of thalassemias to the protein-aggregation disorders will require review of the role of free globin in the pathology of the disease.
- Subjects :
- Adult
Male
Adolescent
Thalassemia
Hemoglobins, Abnormal
Clinical Biochemistry
beta-Globins
High-performance liquid chromatography
03 medical and health sciences
Young Adult
0302 clinical medicine
alpha-Globins
medicine
Humans
gamma-Globins
Amino Acid Sequence
Quadrupole time of flight
Child
Genetics (clinical)
Chromatography, High Pressure Liquid
delta-Globins
Chromatography
Chemistry
Biochemistry (medical)
beta-Thalassemia
Hematology
Hemoglobin Subunits
medicine.disease
Mass spectrometric
030220 oncology & carcinogenesis
Child, Preschool
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Quadrupole
Female
Hemoglobin
030215 immunology
Subjects
Details
- ISSN :
- 1532432X
- Volume :
- 43
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Hemoglobin
- Accession number :
- edsair.doi.dedup.....14640f9742778038b34f386726c432da