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Anti-fibrotic effect of PF2401-SF, a standardized fraction of Salvia miltiorrhiza, in thioacetamide-induced experimental rats liver fibrosis
- Source :
- Archives of Pharmacal Research. 38:549-555
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- We previously reported the in vitro and in vivo hepatoprotective and anti-fibrotic effects of PF2401-SF, a standardized fraction of Salvia miltiorrhiza, against acute and subacute liver injury. The aim of this study was to investigate the effect of PF2401-SF on liver fibrosis induced by thioacetamide (TAA), a chronic liver injury model (12 weeks) that closely resembles fibrosis and cirrhosis in humans. Hepatoprotective activity was indicated by low serum levels of the markers aspartate amino transferase and alanine amino transferase .In addition, compared to the TAA-group livers, the PF2401-SF-treated liver tissues showed no fibrous tissue deposition in the portal areas, hepatocyte morphology more closely resembling normal tissue morphology, and significantly reduced collagen deposition. Furthermore, downregulation of collagen 1(α) and tissue inhibitor of metalloproteinase (TIMP)1 protein and mRNA expression also supports PF2401-SF's anti-fibrotic effect. We also observed reduced expression of α-smooth muscle actin (α-SMA), an important marker of hepatic stellate cells (HSCs) activation. From these results, we conclude that PF2401-SF's anti-fibrotic mechanism in the TAA model involves reduced HSC activation, and may be mediated by downregulation of central markers of fibrosis, including collagen 1(α), TIMP1, and α-SMA.
- Subjects :
- Liver Cirrhosis
Male
Cirrhosis
Salvia miltiorrhiza
Thioacetamide
Pharmacology
Biology
Rats, Sprague-Dawley
chemistry.chemical_compound
Fibrosis
Drug Discovery
medicine
Animals
Liver injury
Plant Extracts
Organic Chemistry
medicine.disease
Rats
Collagen, type I, alpha 1
Treatment Outcome
medicine.anatomical_structure
chemistry
Biochemistry
Hepatocyte
Hepatic stellate cell
Molecular Medicine
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 19763786 and 02536269
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Archives of Pharmacal Research
- Accession number :
- edsair.doi.dedup.....145521ad319ce914d55c1e167e3545f9
- Full Text :
- https://doi.org/10.1007/s12272-014-0425-2