Back to Search
Start Over
Mutation in the Sodium Channel Gene Corresponds With Phenotypic Resistance of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) to Pyrethroids
- Source :
- Journal of medical entomology. 54(6)
- Publication Year :
- 2016
-
Abstract
- The brown dog tick, Rhipicephalus sanguineus sensu lato (Latreille), is a cosmopolitan ectoparasite and vector of pathogens that kill humans and animals. Pyrethroids represent a class of synthetic acaricides that have been used intensely to try to control the brown dog tick and mitigate the risk of tick-borne disease transmission. However, acaricide resistance is an emerging problem in the management of the brown dog tick. Understanding the mechanism of resistance to acaricides, including pyrethroids, is important to adapt brown dog tick control strategies. The main objective of this study was to determine if target-site mutations associated with pyrethroid resistance in other pests could be associated with phenotypic resistance detected in a brown dog tick population from Florida. We amplified segment 6 of the domain III of the voltage-sensitive sodium channel protein, using cDNAs synthesized from pyrethroid-susceptible and pyrethroid-resistant tick strains. A single nucleotide point mutation (SNP) identified in a highly conserved region of domain III S6 in the resistant ticks resulted in an amino acid change from phenylalanine to leucine. This mutation is characteristic of resistance phenotypes in other tick species, and is the first report of this mutation in R. sanguineus. Molecular assays based on this knowledge could be developed to diagnose the risk for pyrethroid resistance, and to inform decisions on integrated brown dog tick management practices.
- Subjects :
- 0301 basic medicine
Insecticides
Rhipicephalus sanguineus
030231 tropical medicine
Population
Voltage-Gated Sodium Channels
Tick
Insecticide Resistance
03 medical and health sciences
0302 clinical medicine
parasitic diseases
Pyrethrins
Animals
Tick Control
Acari
Amino Acid Sequence
education
Genetics
education.field_of_study
General Veterinary
biology
Base Sequence
Acaricide
Knockdown resistance
bacterial infections and mycoses
biology.organism_classification
030104 developmental biology
Infectious Diseases
Phenotype
Insect Science
Mutation
Parasitology
Ixodidae
Subjects
Details
- ISSN :
- 19382928
- Volume :
- 54
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of medical entomology
- Accession number :
- edsair.doi.dedup.....14516ce432f98b99270b77df5e39fe7d