Back to Search
Start Over
Minimum Inhibitory and Minimal Lethal Concentration against Chlamydia trachomatis Dependent on the Time of Addition and the Duration of the Presence of Antibiotics
- Source :
- Chemotherapy. 45:242-248
- Publication Year :
- 1999
- Publisher :
- S. Karger AG, 1999.
-
Abstract
- The purpose of this study was to investigate the properties of several antimicrobial agents found to be effective against Chlamydia trachomatis and to verify the eradication therapy schedule. The in vitro activities of two quinolones (sparfloxacin, ofloxacin), of three macrolides (azithromycin, erythromycin, clarithromycin) and of a tetracycline (doxycycline) against C. trachomatis were evaluated by several methods for the determination of the minimum inhibitory concentration (MIC) and minimal lethal concentration (MLC). MLC of azithromycin was only 2 times higher than that of MIC. On the other hand, MLCs of other antibiotics were 4–16 times higher than their respective MICs. When all antimicrobial agents were added to the infected culture at different times, we found that the quinolones even at a concentration of 64 μg/ml could not inhibit the formation of inclusion if they were added after 20 h from the start of infection. The corresponding period for macrolides and doxycycline was 24 h. When the antibiotics were removed at 8 h after the start of the infection, all antibiotics except azithromycin and clarithromycin were needed at a concentration much higher than their MLCs to inhibit the formation of inclusion. We consider macrolides, especially azithromycin, to be an excellent anti-C. trachomatis drug because of its lower MICs and MLCs values which were also closer together.
- Subjects :
- medicine.drug_class
Antibiotics
Chlamydia trachomatis
Microbial Sensitivity Tests
macromolecular substances
urologic and male genital diseases
Azithromycin
medicine.disease_cause
Microbiology
Lethal Dose 50
Minimum inhibitory concentration
Drug Discovery
Medicine
Pharmacology (medical)
Antibacterial agent
Pharmacology
Doxycycline
4-Quinolones
Minimum bactericidal concentration
business.industry
General Medicine
Antimicrobial
humanities
Anti-Bacterial Agents
Infectious Diseases
Oncology
Macrolides
business
medicine.drug
Subjects
Details
- ISSN :
- 14219794 and 00093157
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Chemotherapy
- Accession number :
- edsair.doi.dedup.....14512cdee387a7e1def75c419e77ef5d