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A simple tube adapter to expedite and automate thawing of viably frozen cells
- Source :
- Journal of immunological methods. 439
- Publication Year :
- 2016
-
Abstract
- Although cryopreserved cell specimens are used throughout biomedical research, the process for thawing samples is labor-intensive and prone to error. Here we describe a small laboratory device that couples an uncapped vial of frozen cells to a conical tube containing warm cell culture media. The entire complex is loaded directly into a centrifuge; within 5min, cells are thawed and diluted out of toxic cryopreservation medium. The recovery and viability of cells are slightly reduced compared to the common (traditional) method. However, antigen-specific T-cell function is not affected. Since no technician time is required (beyond uncapping of vials), our device allows the parallel processing of as many samples as a centrifuge can hold (up to 96, in some models). Moreover, since the samples are not thawed manually in a water bath, the problems associated with technician-to-technician differences in sample handling are minimized, as is the potential for contamination. Importantly, the elimination of substantial labor involving subjective decisions standardizes this process and can reduce variability in results from cryopreserved specimens.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Materials science
Time Factors
Cell Survival
T-Lymphocytes
Immunology
Centrifugation
Vial
Laboratory device
Cryopreservation
03 medical and health sciences
Adapter (genetics)
Cryoprotective Agents
Conical tube
parasitic diseases
medicine
Immunology and Allergy
Humans
Sample handling
Automation, Laboratory
Centrifuge
Equipment Design
Flow Cytometry
Surgery
Culture Media
030104 developmental biology
Cryopreserved Cell
Biomedical engineering
Subjects
Details
- ISSN :
- 18727905
- Volume :
- 439
- Database :
- OpenAIRE
- Journal :
- Journal of immunological methods
- Accession number :
- edsair.doi.dedup.....144e150e16204fd6c0b3e87b5ce3f106