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High-Content Screening for the Detection of Drug-Induced Oxidative Stress in Liver Cells
- Source :
- Antioxidants, Antioxidants, Vol 10, Iss 106, p 106 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Drug-induced liver injury (DILI) remains a major cause of drug development failure, post-marketing warnings and restriction of use. An improved understanding of the mechanisms underlying DILI is required for better drug design and development. Enhanced reactive oxygen species (ROS) levels may cause a wide spectrum of oxidative damage, which has been described as a major mechanism implicated in DILI. Several cell-based assays have been developed as in vitro tools for early safety risk assessments. Among them, high-content screening technology has been used for the identification of modes of action, the determination of the level of injury and the discovery of predictive biomarkers for the safety assessment of compounds. In this paper, we review the value of in vitro high-content screening studies and evaluate how to assess oxidative stress induced by drugs in hepatic cells, demonstrating the detection of pre-lethal mechanisms of DILI as a powerful tool in human toxicology.
- Subjects :
- 0301 basic medicine
Drug
hepatotoxicity
Physiology
media_common.quotation_subject
Clinical Biochemistry
Review
medicine.disease_cause
Bioinformatics
high-content screening
Biochemistry
03 medical and health sciences
0302 clinical medicine
Medicine
oxidative stress
Molecular Biology
media_common
chemistry.chemical_classification
Liver injury
Reactive oxygen species
business.industry
Mechanism (biology)
lcsh:RM1-950
Cell Biology
medicine.disease
cell models
mitochondria
lcsh:Therapeutics. Pharmacology
030104 developmental biology
Drug development
chemistry
030220 oncology & carcinogenesis
High-content screening
Hepatic stellate cell
business
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Antioxidants
- Accession number :
- edsair.doi.dedup.....14242b1672f666ff77eb360fc0109449