Back to Search
Start Over
Response of the human hepatic tissue cultures Hep-G2 and WRL-68 to cocaine
- Source :
- Journal of pharmacological and toxicological methods. 33(2)
- Publication Year :
- 1995
-
Abstract
- The hydrolytic metabolism of cocaine into benzoylecgonine, ecgonine methyl ester, and ecgonine was studied in the human hepatoma cell line Hep-G2 and in the nontumorigenic fetal hepatic cell line WRL-68. Also, the toxicological response of these cells to cocaine was compared to previously published results obtained with perfused liver cells and in vivo systems. Our experiments indicated that Hep-G2 appear to have similar metabolic and toxicological patterns to in vivo and perfused cell systems. The WRL-68 tissue culture system was found to be less similar. These results suggest Hep-G2 cells can be utilized to study cocaine metabolism and toxicology, and possibly in studies involving other xenobiotic compounds.
- Subjects :
- Carcinoma, Hepatocellular
Chromatography, Gas
Cell
Pharmacology
Biology
Toxicology
Mass Spectrometry
Cell Line
chemistry.chemical_compound
Tissue culture
Cocaine
In vivo
medicine
Tumor Cells, Cultured
Humans
Cell Death
Hep G2
medicine.anatomical_structure
chemistry
Liver
Hepatic stellate cell
Benzoylecgonine
Ecgonine
Xenobiotic
Cell Division
Subjects
Details
- ISSN :
- 10568719
- Volume :
- 33
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of pharmacological and toxicological methods
- Accession number :
- edsair.doi.dedup.....1423032c49956152481fd2bdc8f84e71