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Characterising genes associated with flowering time in carrot (Daucus carotaL.) using transcriptome analysis
- Source :
- Plant Biology. 19:286-297
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Carrot is generally regarded as a biennial plant with an obligatory vernalization requirement. Early spring cultivation makes plants vulnerable to premature bolting, which results in a loss of commercial value. However, our knowledge of flowering time genes and flowering mechanisms in carrot remain limited. Bolting behavior of D. carota ssp. carota 'Songzi', a wild species sensitive to flower induction by vernalization and photoperiod, and orange cultivar 'Amsterdam forcing', and their offspring were investigated in different growing conditions. We performed RNA-seq to identify the flowering time genes, and digital gene expression (DGE) analysis to examine their expression levels. The circadian patterns of related genes were identified by qPCR. The results showed bolting behavior of carrot was influenced by low temperature, illumination intensity and photoperiod. A total of 45 flowering time-related unigenes were identified, which were classified into five categories including photoperiod, vernalization, autonomous and gibberellin pathway, and floral integrators. Homologs of LATE ELONGATED HYPOCOTYL (LHY) and CONSTANS-LIKE 2 (COL2) were more highly expressed under short day condition than under long day condition. Homologs of COL2, CONSTANS-LIKE 5 (COL5), SUPPRESSION OF OVEREXPRESSION OF CONSTANS 1 (SOC1), FLOWERING LOCUS C (FLC) and GIBBERELLIC ACID INSENSITIVE (GAI) were differentially expressed between 'Songzi' and 'Amsterdam forcing'. The homolog of COL2 (Dct43207) was repressed by light, but that of COL5 (Dct20940) was induced. A preliminary model of genetic network controlling flowering time was constructed by associating the results of DGE analysis with correlation coefficients between genes. This study provides useful information for further investigating the genetic mechanism of flowering in carrot.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Time Factors
Light
Photoperiod
Flowers
Plant Science
Biology
01 natural sciences
03 medical and health sciences
Gene Expression Regulation, Plant
Circadian Clocks
Flower induction
Botany
Flowering Locus C
Gene Regulatory Networks
Phylogeny
Ecology, Evolution, Behavior and Systematics
photoperiodism
Bolting
Gene Expression Profiling
fungi
food and beverages
General Medicine
Vernalization
biology.organism_classification
Daucus carota
030104 developmental biology
Gibberellin
Biennial plant
Transcriptome
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14358603
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Plant Biology
- Accession number :
- edsair.doi.dedup.....1414a974706e302761388006a3664cc4