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Lactic acid bacteria and bifidobacteria attenuate the proinflammatory response in intestinal epithelial cells induced by Salmonella enterica serovar Typhimurium
- Source :
- Canadian journal of microbiology. 59(1)
- Publication Year :
- 2013
-
Abstract
- Inflammation is a physiological response to infections and tissue injury; however, abnormal immune responses can give rise to chronic inflammation and contribute to disease progression. Various dietary components, including probiotic lactic acid bacteria and prebiotics, have the potential to modulate intestinal inflammatory responses. One factor in particular, the chemokine interleukin-8 (IL-8, CXCL-8), is one of the major mediators of the inflammatory response. The purpose of this study was to investigate modulation of the inflammatory host response induced by Salmonella enterica serovar Typhimurium DT104 in the presence of selected probiotics and lactic acid bacteria (LAB) isolated from human sources, dairy products, and farm animals. IL-8 gene expression and protein production in HT-29 cells were evaluated by real-time PCR and ELISA, respectively. Pre-incubation of HT-29 cells with Lactobacillus kefir IM002, Bifidobacterium adolescentis FRP 61, Bifidobacterium longum FRP 68 and FRP 69, Bifidobacterium breve FRP 334, and Leuconostoc mesenteroides IM080 significantly inhibited IL-8 secretion induced by Salmonella Typhimurium DT104. Co-culture of selected probiotics and Salmonella Typhimurium DT104 reduced IL-8 production, while potential probiotics and LAB had no effect on IL-8 secretion in HT-29 cells preincubated with Salmonella Typhimurium DT104 prior to adding probiotics. Lactobacillus kefir IM002 supernatant also significantly reduced IL-8 production. In conclusion, our study suggests that probiotic bifidobacteria and LAB modulate cytokine induction and possess anti-inflammatory properties; however, the effectiveness is strain dependent.
- Subjects :
- Salmonella typhimurium
Salmonella
Bifidobacterium longum
Immunology
ved/biology.organism_classification_rank.species
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Real-Time Polymerase Chain Reaction
Applied Microbiology and Biotechnology
Microbiology
law.invention
Proinflammatory cytokine
Probiotic
Immune system
law
Genetics
medicine
Humans
Molecular Biology
Inflammation
Bifidobacterium breve
biology
ved/biology
Probiotics
Interleukin-8
food and beverages
Epithelial Cells
General Medicine
biology.organism_classification
Intestines
Lactobacillus
Gene Expression Regulation
Salmonella enterica
Salmonella Infections
Cytokines
Bifidobacterium
HT29 Cells
Bacteria
Subjects
Details
- ISSN :
- 14803275
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Canadian journal of microbiology
- Accession number :
- edsair.doi.dedup.....140016a6080bf5cb75bcba280bf18b73