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A novel human leiomyoma tissue derived matrix for cell culture studies
- Source :
- BMC Cancer
- Publication Year :
- 2015
-
Abstract
- Background The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel. Methods A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. Results We demonstrated that only 34 % of Myogel’s molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® in in vitro Transwell® invasion and capillary formation assays. Conclusions In conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-1944-z) contains supplementary material, which is available to authorized users.
- Subjects :
- Pathology
Cancer Research
INVASION
Cell Culture Techniques
Biocompatible Materials
Mass Spectrometry
Extracellular matrix
0302 clinical medicine
Electrophoresis, Gel, Two-Dimensional
Migration
0303 health sciences
Leiomyoma
Sepharose
3. Good health
Cell biology
Extracellular Matrix
Oncology
030220 oncology & carcinogenesis
Uterine Neoplasms
Spheroid formation
GROWTH
Electrophoresis, Polyacrylamide Gel
Female
TUMOR MICROENVIRONMENT
Capillary formation
Research Article
EXPRESSION
medicine.medical_specialty
PROTEINS
3122 Cancers
Biology
Tumor microenvironment matrix
Hanging drop
03 medical and health sciences
Gentamicin protection assay
medicine
Genetics
EXTRACELLULAR-MATRIX
Humans
CANCER-CELLS
030304 developmental biology
Tumor microenvironment
IDENTIFICATION
Spheroid
Colony formation
IN-VITRO
medicine.disease
In vitro
MODEL
Cell culture
Cancer cell
Gels
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....13ef7edef3e4764f2168d571c3e8c5c8