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Depletion of c-Myc inhibits human colon cancer colo 320 cells' growth
- Source :
- Cancer biotherapyradiopharmaceuticals. 23(2)
- Publication Year :
- 2008
-
Abstract
- Human colon cancer is the leading cause of cancer death in both men and women worldwide. The c-Myc gene is frequently deregulated and overexpressed in this malignancy, and strategies designed to inhibit c-Myc expression in cancer cells may have considerable therapeutic value. We design and use short hairpin RNA (shRNA) to inhibit c-Myc expression in Colo 320 cells and validat its effect on cell proliferation. In this study, four c-Myc-shRNA expression vectors were constructed and introduced into Colo 320 cells, and the cell cycle and apoptotic cells were analyzed by flow cytometry. The effects of c-Myc silencing on tumor-cell growth was assessed by the soft agar assay and by DNA synthesis experiments. Expression of c-Myc was also assessed by real-time reverse transcription polymerase chain reaction and Western blot analysis. Upon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited cell growth and induced apoptosis in Colo 320 cells. c-Myc downregulation also increased cell population in the G0-G1 phase. In conclusion, our findings demonstrate that shRNA can inhibit the DNA replication and induce apoptosis in Colo 320 cells effectively and, therefore, could be used as a new potential anticancer tool for the therapy of human colon cancer.
- Subjects :
- Cancer Research
Cell
Genetic Vectors
Molecular Sequence Data
Down-Regulation
Biology
Small hairpin RNA
Proto-Oncogene Proteins c-myc
Cell Line, Tumor
medicine
Gene silencing
Humans
Radiology, Nuclear Medicine and imaging
Cell Proliferation
Pharmacology
Base Sequence
Cell growth
General Medicine
Cell cycle
Molecular biology
Reverse transcription polymerase chain reaction
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Oncology
Apoptosis
Cancer cell
Colonic Neoplasms
RNA Interference
Subjects
Details
- ISSN :
- 15578852
- Volume :
- 23
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cancer biotherapyradiopharmaceuticals
- Accession number :
- edsair.doi.dedup.....13e91fba366206f33e26a8092e83504a