Phenolic Group of α-Tocopherol Anchors at the Lipid-Water Interface of Fully Saturated Membranes

α-Tocopherol is considered to carry on a very important role as antioxidant of membranes and lipoproteins and other biological roles as membrane stabilizers and bioactive lipids. Given its essential role, it is very important to fully understand its location in the membrane. In this work, the vertical location of vitamin E in saturated membranes has been studied using biophysical techniques. Small- and wide-angle X-ray diffraction experiments show that α-tocopherol alters the water layer between bilayers in both DMPC and DPPC, indicating its proximity to this surface. The quenching of the intrinsic fluorescence of α-tocopherol indicates a low quenching efficiency by acrylamide and a higher quenching by 5-doxyl-PC than by 9- and 16-doxyl-PC. These results suggest that in both DMPC and DPPC membranes the chromanol ring is not far away from the surface of the membrane but within the bilayer. 1H-NMR-NOESY MAS-NMR studies showed that α-tocopherol is localized similarly in DMPC and DPPC membranes, with the chro...