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Attenuated lipopolysaccharide-induced inflammatory bladder hypersensitivity in mice deficient of transient receptor potential ankilin1
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Nature America, Inc, 2018.
-
Abstract
- Transient receptor potential ankyrin 1 (TRPA1) channel expressed by urothelial cells and bladder sensory nerve fibers might act as a bladder mechanosensor and nociceptive transducer. To disclose the role of TRPA1 in bladder function and inflammation-associated hypersensitivity, we evaluated in vitro and in vivo bladder function and inflammatory mechanosensory and nociceptive responses to intravesical lipopolysaccharide (LPS)-instillation in wild type (WT) and TRPA1-knock out (KO) mice. At baseline before treatment, no significant differences were observed in frequency volume variables, in vitro detrusor contractility, and cystometric parameters between the two groups in either sex. LPS-instillation significantly increased voiding frequency and decreased mean voided volume at 24–48 hours after instillation in WT but not in TRPA1-KO mice. LPS-instillation also significantly increased the number of pain-like behavior at 24 hours after instillation in WT mice, but not in TRPA1-KO mice. Cystometry 24 hours after LPS-instillation revealed shorter inter-contraction intervals in the WT mice compared with TRPA1-KO mice. In contrast, inflammatory cell infiltration in the bladder suburothelial layer was not significantly different between the two groups. These results indicate that TRPA1 channels are involved in bladder mechanosensory and nociceptive hypersensitivity accompanied with inflammation but not in physiological bladder function or development of bladder inflammation.
- Subjects :
- Lipopolysaccharides
Male
medicine.medical_specialty
Lipopolysaccharide
Urinary Bladder
030232 urology & nephrology
lcsh:Medicine
Inflammation
urologic and male genital diseases
Article
Mice
03 medical and health sciences
Transient receptor potential channel
chemistry.chemical_compound
0302 clinical medicine
In vivo
Internal medicine
Animals
Medicine
lcsh:Science
TRPA1 Cation Channel
Mice, Knockout
Multidisciplinary
medicine.diagnostic_test
business.industry
lcsh:R
Urinary Bladder Diseases
Wild type
Cystometry
food and beverages
female genital diseases and pregnancy complications
Mice, Inbred C57BL
medicine.anatomical_structure
Endocrinology
Nociception
chemistry
lcsh:Q
Female
Urothelium
medicine.symptom
business
030217 neurology & neurosurgery
psychological phenomena and processes
Sensory nerve
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....13bacdf2d2740f37054e9369ef176821