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Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 286:G458-G466
- Publication Year :
- 2004
- Publisher :
- American Physiological Society, 2004.
-
Abstract
- It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small intestine were fluorescence labeled, and their adhesion to mucosal microvessels was observed by intravital microscopy. Lamina proprial lymphocytes (LPL) and intraepithelial lymphocytes (IEL) adhered to both the small intestine and colon, and desensitization of CCR9 with TECK/CCL25 or anti-TECK/CCL25 antibody significantly inhibited these adhesions only in small intestine. At both sites, TNF-α significantly increased LPL adhesion but not IEL adhesion. Desensitization of CCR9 or anti-TECK/CCL25 antibody also attenuated the TNF-α-induced LPL adhesion in the small intestine. Increased expression of TECK/CCL25 by TNF-α was observed in the lamina propria of small intestine. TECK/CCL25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions.
- Subjects :
- Colon
Physiology
T-Lymphocytes
medicine.medical_treatment
Blotting, Western
CCR9
Receptors, Cell Surface
Biology
Epithelium
Mice
Receptors, CCR
Intestinal mucosa
Cell Movement
Physiology (medical)
Intestine, Small
medicine
Animals
Endothelium
Intestinal Mucosa
Mice, Inbred BALB C
Hepatology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Gastroenterology
T lymphocyte
Flow Cytometry
Immunohistochemistry
Enteritis
Gut-specific homing
Cytokine
Microscopy, Fluorescence
Chemokines, CC
Immunology
Intraepithelial lymphocyte
Female
Receptors, Chemokine
CCL25
Homing (hematopoietic)
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....13796fb498c11c7f91a87fe19cbb487c