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Effects of high fat diet on lipid accumulation, oxidative stress and autophagy in the liver of Chinese softshell turtle (Pelodiscus sinensis)
- Source :
- Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 240:110331
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The present study was performed to determine the effect of high fat diet in lipid accumulation, oxidative stress and autophagy, and to explore the underlying molecular mechanism of high fat diet induced hepatic oxidative damage in Chinese softshell turtle. To this end, the control group were fed a normal fat diet (NFD, 6.38% lipid) and the experimental group were bred high fat diet (HFD, 13.89% lipid) for eight weeks. Lipid accumulation, oxidative stress and autophagy, as well as the mRNA expression of genes related to the antioxidant system were determined in the liver. Results showed that high fat diet not only exacerbated lipid accumulation in the liver and serum through increasing contents of triglyceride, total cholesterol and low-density lipoprotein and decreasing content of high-density lipoprotein, but also induced liver injury through increasing activities of alanine aminotransferase and aspartate aminotransferase in the serum. In addition, the experimental subject induced oxidative injury for the increase of reactive oxygen species, malondialdehyde and protein carbonyl contents and the reduction of glutathione contents, anti-superoxide anion capacity and catalase, total superoxide dismutase, glutathione peroxidase, glutathione-S transferase activities. Meanwhile, antioxidant-related signaling molecule expression were also decreased, which might attribute to regulate antioxidant-related signaling molecule. On top of that, it indicated promote the occurrence of liver autophagy via up-regulating expressions of AMP activated protein kinase, UNC-51-like kinase 1, Microtubule-associated proteins 1A/1B light chain 3 and down-regulating gene expression of mammalian target of rapamycin. In conclusion, high fat diet could enhance lipid accumulation in the liver and serum, lead to liver injury and oxidative damage, impair liver antioxidant capacity, regulate antioxidant-related signaling molecule expression and activate hepatic autophagy.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Antioxidant
Physiology
Autophagic Cell Death
medicine.medical_treatment
010501 environmental sciences
Biology
medicine.disease_cause
01 natural sciences
Biochemistry
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
medicine
Animals
Molecular Biology
0105 earth and related environmental sciences
chemistry.chemical_classification
Liver injury
Reactive oxygen species
Glutathione peroxidase
Glutathione
Lipid Metabolism
Malondialdehyde
medicine.disease
Dietary Fats
Turtles
Oxidative Stress
030104 developmental biology
Endocrinology
Liver
chemistry
biology.protein
Oxidative stress
Subjects
Details
- ISSN :
- 10964959
- Volume :
- 240
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
- Accession number :
- edsair.doi.dedup.....13755af26fcfff8f9491ac33470318a5