Additional file 10 of CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in Huntington’s disease

Additional file 10. Alteration in CK2α’ expression changes cytokine profiles but not microglia. a, RT-qPCR analysis for CK2α’ and IL-6 in STHdhQ7/Q7 (control) and STHdhQ111/Q111 (HD) cell (n = 5 independent experiments). Data were normalized to GAPDH and control cells. b, siRNA knockdown of CK2α’ for 24 h in STHdhQ111/Q111 cells and RT-qPCR. Data were normalized with GAPDH and relativized to non-targeting control siRNA-treated cells (scramble), (n = 6 independent experiments). c, d, Representative images of mouse cytokine array panels from striatum extracts of WT, zQ175, and zQ175:CK2α’(+/-) at 12-14 months of age (n = 6 mice/genotype). e, f, Iba1 immunoblotting in the striatum of 12 months old WT, zQ175 and zQ175:CK2α’, quantification was measured by image analyses using Image J software, GAPDH is used as a loading marker. g-i, Representative images show the labeling of Iba1 in the dorsal striatum of WT, zQ175 and zQ175:CK2α’(+/-) at 3, 6 and 12 months old (g). Images were analyzed using the Image J software. Scale bar, 100 µm. Iba1+ cell body area in 12 months old mice (n = 3-4 mice/genotype, 18 images averaged/mouse) (h) and percent of Iba1+ cells in 300mm2 (n=3-4 mice/genotype, at least 9 images averaged/mouse) (i). Error bars denote mean ± SEM, values were analyzed by Student's t test in d, and one-way ANOVA and Tukey post-hoc test in a, b, f, h, i. *p represent p-values comparing zQ175 and WT, #p are p-values comparing zQ175 and zQ175:CK2α’(+/-).