Supplementary Figures 1 - 4, Tables 1 - 3 from Essential Role of Aldehyde Dehydrogenase 1A3 for the Maintenance of Non–Small Cell Lung Cancer Stem Cells Is Associated with the STAT3 Pathway

PDF file - 465K, Supplementary Table S1. The correlation between ALDH1A3 protein expression and percentage of ALDH+ cells in NSCLC lines (Data for Figure 2D). Supplementary Table S2. Incidence of xenograft tumors derived from control or ALDH1A3 knockdown lung cancer cells. Supplementary Table S3. The association between ALDH1A3 protein expression and clinicopathological variables for 455 NSCLC patient samples. Figure S1. ALDH profiling of 8 NSCLC lines using the flow cytometry based Aldefluor assay. Figure S2. Anchorage dependent colony formation efficiency was determined by growing serial dilutions of sorted ALDH+ and ALDH- cells for 2 weeks in liquid culture. Figure S3. A, ALDH1A3 protein expression in a set of NSCLC lines was detected by western blots. The corresponding percentage of ALDH+ cells was analyzed by Aldefluor assay. B, western blot showed ALDH1A1 protein expression in certain HBEC and NSCLC lines. C, ALDH1A3 was knocked down using three siRNAs in a panel of NSCLC lines with different driver mutations followed by liquid colony formation assays. RT-PCR was performed to examine ALDH1A3 mRNA expression. Figure S4. Ectopic expression of ALDH1A3 does not affect tumorigenicity of lung cancer cells.