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ELISA for Quantitative Determination of Hepatitis B Virus Surface Antigen
- Source :
- Immune Network
- Publication Year :
- 2017
- Publisher :
- The Korean Association of Immunologists, 2017.
-
Abstract
- Several studies have reported a good correlation between levels of serum hepatitis B virus surface antigen (HBsAg) and covalently closed circular DNA (cccDNA) before and after antiviral therapy. As a result, the quantification of HBsAg levels has attracted much attention in recent years as an important approach to evaluate viral activity. In this study, mAbs against HBsAg were generated and 9 mAbs (H17, H30, H31, H67, H73, H97, H101, H118, and H128) were investigated for optimization of HBsAg quantitation ELISA. Determination of the best combinations of mAbs for sandwich ELISA identified H17 and H31 mAbs as the ideal capture and horseradish peroxidase (HRP) conjugate mAbs, respectively. A standard curve for the current assay system exhibited linearity up to 40 ng/ml of HBsAg while a detection limit of approximately 1 ng/ml of HBsAg was also estimated, which was comparable to that of the other commercial ELISA kits. The ELISA system established in this study is particularly differentiated from other commercial kits in using mAbs for both capture and HRP conjugate, which provides a solution to inconsistency of quality and ethical issues in polyclonal antibodies production using laboratory animals.
- Subjects :
- 0301 basic medicine
Monoclonal antibody
HBsAg
medicine.drug_class
Immunology
Brief Communication
Horseradish peroxidase
03 medical and health sciences
0302 clinical medicine
Quantification
medicine
Immunology and Allergy
Detection limit
biology
Chemistry
cccDNA
Virology
Standard curve
030104 developmental biology
Infectious Diseases
Polyclonal antibodies
Hepatitis B virus surface antigen
biology.protein
030211 gastroenterology & hepatology
ELISA
Conjugate
Subjects
Details
- Language :
- English
- ISSN :
- 20926685 and 15982629
- Volume :
- 17
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Immune Network
- Accession number :
- edsair.doi.dedup.....1369ea7a07f3da3ca0b04f18e81dba61