Additional file 1 of Efficient biosynthesis of (R)-mandelic acid from styrene oxide by an adaptive evolutionary Gluconobacter oxydans STA

Additional file 1: Fig. S1 (a) SDS–PAGE of SpEH expression strains. Lane M: protein marker; Lane 1: whole cell protein of wild-type G. oxydans without overexpressing SpEH; Lane 2: whole cell protein of WT-SpEH; Lane 3: whole cell protein of G. oxydans STA without overexpressing SpEH; Lane 4: whole cell protein of STA-SpEH. (b) Enzyme activity of crude SpEH of WT-SpEH and STA-SpEH. Fig. S2. Membrane permeability and hydrophobicity analysis of G. oxydans STA strains. (a) NPN fluorescence intensity analyses of outer membrane permeability in STA stains. (b) Inner membrane permeability change of propidium iodide (PI) uptake factor in STA stains. (c) Membrane hydrophobicity was changed STA stains. **P < 0.01. ***P < 0.001. Table S1. Strains used in this study. Table S2. Primers used in this study. Table S3. Gene IDs and protein functions of promoters.