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A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products
- Source :
- Molecules, Molecules, Vol 24, Iss 11, p 2180 (2019)
- Publication Year :
- 2019
-
Abstract
- Silybin (SBN) is a major active constituent of silymarin, a mixture of flavonoids found in fruits and seeds of milk thistle. The aim of this study was to describe a simple bioanalytical method for quantifying SBN in rat plasma. A simple protein deproteinization procedure with acetonitrile (ACN) was employed for plasma sample preparation. A reversed column and gradient elution of a mobile phase (mixture of phosphate buffer (pH 5.0) and ACN) were used for chromatographic separation. The selectivity, linearity (50–5000 ng/mL), precision, accuracy, recovery, matrix effect, and stability for this method were validated as per the current Food and Drug Administration (FDA) guidelines. Our method for SBN was applied to a comparative pharmacokinetic study on four different commercial silymarin products. This in vivo rat study demonstrated that product #4 significantly enhanced the relative oral bioavailability of SBN, as compared to product #1–3. Therefore, the bioanalytical method proposed herein could serve as a promising alternative for preclinical pharmacokinetic studies on silymarin products and, by extension, clinical use after partial modification and validation.
- Subjects :
- Male
Bioanalysis
Time Factors
silymarin product
Pharmaceutical Science
Administration, Oral
02 engineering and technology
comparative pharmacokinetics
01 natural sciences
High-performance liquid chromatography
silybin
Analytical Chemistry
lcsh:QD241-441
Rats, Sprague-Dawley
lcsh:Organic chemistry
Pharmacokinetics
In vivo
Drug Discovery
Animals
rat
Physical and Theoretical Chemistry
Hplc method
Chromatography, High Pressure Liquid
Chromatography
Milk Thistle
Chemistry
Communication
010401 analytical chemistry
Organic Chemistry
Reference Standards
021001 nanoscience & nanotechnology
Quantitative determination
0104 chemical sciences
Bioavailability
Chemistry (miscellaneous)
Molecular Medicine
HPLC
0210 nano-technology
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....135913397b1f4c8fe0cab6d359ef565d