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A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products

Authors :
Seon-Kwang Lee
Eun-Sol Ha
Ji-Min Kim
Woo-Yong Sim
Min-Soo Kim
Seong-Wook Seo
Dong-Gyun Han
In-Soo Yoon
Source :
Molecules, Molecules, Vol 24, Iss 11, p 2180 (2019)
Publication Year :
2019

Abstract

Silybin (SBN) is a major active constituent of silymarin, a mixture of flavonoids found in fruits and seeds of milk thistle. The aim of this study was to describe a simple bioanalytical method for quantifying SBN in rat plasma. A simple protein deproteinization procedure with acetonitrile (ACN) was employed for plasma sample preparation. A reversed column and gradient elution of a mobile phase (mixture of phosphate buffer (pH 5.0) and ACN) were used for chromatographic separation. The selectivity, linearity (50–5000 ng/mL), precision, accuracy, recovery, matrix effect, and stability for this method were validated as per the current Food and Drug Administration (FDA) guidelines. Our method for SBN was applied to a comparative pharmacokinetic study on four different commercial silymarin products. This in vivo rat study demonstrated that product #4 significantly enhanced the relative oral bioavailability of SBN, as compared to product #1–3. Therefore, the bioanalytical method proposed herein could serve as a promising alternative for preclinical pharmacokinetic studies on silymarin products and, by extension, clinical use after partial modification and validation.

Details

ISSN :
14203049
Volume :
24
Issue :
11
Database :
OpenAIRE
Journal :
Molecules (Basel, Switzerland)
Accession number :
edsair.doi.dedup.....135913397b1f4c8fe0cab6d359ef565d