Back to Search
Start Over
Upregulation of myosin Va by Snail is involved in cancer cell migration and metastasis
- Source :
- International Journal of Cancer. 126:53-64
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- Cell migration, which involves acto-myosin dynamics, cell adhesion, membrane trafficking and signal transduction, is a prerequisite for cancer cell metastasis. Here, we report that an actin-dependent molecular motor, unconventional myosin Va, is involved in this process and implicated in cancer metastasis. The mRNA expression of myosin Va is increased in a number of highly metastatic cancer cell lines and metastatic colorectal cancer tissues. Suppressing the expression of myosin Va by lentivirus-based RNA interference in highly metastatic cancer cells impeded their migration and metastasis capabilities both in vitro and in vivo. In addition, the levels of myosin Va in cancer cell lines are positively correlated with the expression of Snail, a transcriptional repressor that triggers epithelial-mesenchymal transition. Repression or overexpression of Snail in cancer cells caused reduced or elevated levels of myosin Va, respectively. Furthermore, Snail can bind to an E-box of the myosin Va promoter and induce its activity, which indicates that Snail might act as a transcriptional activator. These data demonstrate an essential role of myosin Va in cancer cell migration and metastasis, and suggest a novel target for Snail in its regulation of cancer progression.
- Subjects :
- Chromatin Immunoprecipitation
Cancer Research
Myosin Type V
Electrophoretic Mobility Shift Assay
macromolecular substances
Snail
Biology
Metastasis
Cell Movement
Cell Line, Tumor
biology.animal
Myosin
medicine
Humans
Neoplasm Metastasis
RNA, Small Interfering
Promoter Regions, Genetic
Cell adhesion
DNA Primers
Base Sequence
Myosin Heavy Chains
Reverse Transcriptase Polymerase Chain Reaction
Cancer
Cell migration
medicine.disease
Up-Regulation
Oncology
Immunology
Cancer cell
Cancer research
RNA Interference
Snail Family Transcription Factors
Signal transduction
Transcription Factors
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....1340f25a140a15dc322bc3637060de22