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External application of nitrogen alleviates toxicity of cadmium on poplars via starch and sucrose metabolism
- Source :
- Tree Physiology. 41:2126-2141
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Phytoremediation technology can help achieve moderate cost and considerable effect with respect to the remediation of heavy metal (HM) pollution in soil and water. Many previous studies have suggested the role of nitrogen (N) in the alleviation of effects of HM on plants. Herein, we sought to determine the molecular mechanisms by which additional N supplementation mitigates cadmium (Cd) toxicity in poplars using a combination of physiological, transcriptomic and phosphoproteomic analyses. The application of N can alleviate the toxicity of Cd to Populus by reducing chlorophyll degradation, maintaining the stability of ions inside and outside the cell membrane and increasing the soluble sugar content. Plant samples from the control, Cd stress and Cd_N treatments were used for an integrated analysis of the transcriptome, as well as for phosphoproteomics analysis. Moreover, 1314 differentially expressed genes and 119 differentially expressed kinase genes were discovered. Application of additional N under Cd stress promoted the phosphorylation process. Furthermore, 51 significantly enriched phosphorylated protein sites and 23 differentially expressed kinases were identified using phosphoproteomic and proteomic analyses. Importantly, transcriptomic and phosphoproteomic analyses jointly determined that the application of N could activate corresponding gene expression [UDP-glucose-dehydrogenase (UGD), GAUT, PME, pectin lyase, UDP-glucose-pyrophosphorylase 2 (UGP2), sucrose phosphate synthase (SPS), SUS and SPP2] and protein phosphorylation (UGP2 and SPS) in the sugar and starch synthesis pathways, which promoted the synthesis of sucrose and soluble sugar and subsequently alleviated the damage caused by Cd.
- Subjects :
- Proteomics
0106 biological sciences
0301 basic medicine
Sucrose
Nitrogen
Physiology
Plant Science
Plant Roots
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Protein phosphorylation
Sugar
Pectin lyase
biology
Chemistry
Kinase
Phosphoproteomics
Starch
Populus
030104 developmental biology
Biochemistry
biology.protein
Phosphorylation
Sucrose-phosphate synthase
Cadmium
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17584469
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Tree Physiology
- Accession number :
- edsair.doi.dedup.....13252097e0b0fac4d0977a6a41857627