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Distinct spatiotemporal expression of EFA6D, a guanine nucleotide exchange factor for ARF6, among the EFA6 family in mouse brain
- Source :
- Brain Research. 1093:1-11
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- The EFA6 family is a member of guanine nucleotide exchange factors (GEFs) that can activate ARF6 specifically in vitro. In this study, we determined the complete primary sequence of mouse EFA6D encoding a protein of 1004 amino acids with a calculated molecular weight of 111,207 Da. In ARF pull-down assay, EFA6D showed a preferential GEF activity toward ARF6. RT-PCR analysis revealed the widespread tissue distribution of EFA6D and the high expression of EFA6A, C and D in the brain. In situ hybridization analysis demonstrated a distinct spatiotemporal expression pattern of EFA6D from those of EFA6A and C in mouse brain. Furthermore, immunoblot analysis revealed that EFA6D was highly concentrated in the postsynaptic density fraction. These findings suggest differential spatiotemporal regulation of ARF6 by three members of the EFA6 family in the brain.
- Subjects :
- ADP ribosylation factor
Immunoblotting
Molecular Sequence Data
In situ hybridization
Biology
Mice
Immunoblot Analysis
Animals
Guanine Nucleotide Exchange Factors
Protein Isoforms
Amino Acid Sequence
RNA, Messenger
Molecular Biology
Peptide sequence
In Situ Hybridization
chemistry.chemical_classification
Sequence Homology, Amino Acid
ADP-Ribosylation Factors
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Brain
Molecular biology
In vitro
Rats
Amino acid
chemistry
ADP-Ribosylation Factor 6
Neurology (clinical)
Guanine nucleotide exchange factor
Postsynaptic density
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1093
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....131df2e2c939328bea417db807d25d77