Back to Search
Start Over
Autophagy regulates glucose-mediated root meristem activity by modulating ROS production in Arabidopsis
- Source :
- Autophagy
- Publication Year :
- 2018
-
Abstract
- Glucose produced from photosynthesis is a key nutrient signal regulating root meristem activity in plants; however, the underlying mechanisms remain poorly understood. Here, we show that, by modulating reactive oxygen species (ROS) levels, the conserved macroautophagy/autophagy degradation pathway contributes to glucose-regulated root meristem maintenance. In Arabidopsis thaliana roots, a short exposure to elevated glucose temporarily suppresses constitutive autophagosome formation. The autophagy-defective autophagy-related gene (atg) mutants have enhanced tolerance to glucose, established downstream of the glucose sensors, and accumulate less glucose-induced ROS in the root tips. Moreover, the enhanced root meristem activities in the atg mutants are associated with improved auxin gradients and auxin responses. By acting with AT4G39850/ABCD1 (ATP-binding cassette D1; Formerly PXA1/peroxisomal ABC transporter 1), autophagy plays an indispensable role in the glucose-promoted degradation of root peroxisomes, and the atg mutant phenotype is partially rescued by the overexpression of ABCD1. Together, our findings suggest that autophagy is an essential mechanism for glucose-mediated maintenance of the root meristem. Abbreviation: ABA: abscisic acid; ABCD1: ATP-binding cassette D1; ABO: ABA overly sensitive; AsA: ascorbic acid; ATG: autophagy related; CFP: cyan fluorescent protein; Co-IP: co-immunoprecipitation; DAB: 3’,3’-diaininobenzidine; DCFH-DA: 2’,7’-dichlorodihydrofluorescin diacetate; DR5: a synthetic auxin response element consists of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element; DZ: differentiation zone; EZ, elongation zone; GFP, green fluorescent protein; GSH, glutathione; GUS: β-glucuronidase; HXK1: hexokinase 1; H2O2: hydrogen peroxide; IAA: indole-3-acetic acid; IBA: indole-3-butyric acid; KIN10/11: SNF1 kinase homolog 10/11; MDC: monodansylcadaverine; MS: Murashige and Skoog; MZ: meristem zone; NBT: nitroblue tetrazolium; NPA: 1-N-naphtylphthalamic acid; OxIAA: 2-oxindole-3-acetic acid; PIN: PIN-FORMED; PLT: PLETHORA; QC: quiescent center; RGS1: Regulator of G-protein signaling 1; ROS: reactive oxygen species; SCR: SCARECROW; SHR, SHORT-ROOT; SKL: Ser-Lys-Leu; SnRK1: SNF1-related kinase 1; TOR: target of rapamycin; UPB1: UPBEAT1; WOX5: WUSCHEL related homeobox 5; Y2H: yeast two-hybrid; YFP: yellow fluorescent protein
- Subjects :
- 0301 basic medicine
Yellow fluorescent protein
Meristem
Arabidopsis
Autophagy-Related Proteins
ATP Binding Cassette Transporter, Subfamily D, Member 1
Plant Roots
03 medical and health sciences
chemistry.chemical_compound
Auxin
Gene Expression Regulation, Plant
Autophagy
Peroxisomes
Arabidopsis thaliana
peroxisome
Molecular Biology
Abscisic acid
chemistry.chemical_classification
reactive oxygen species
030102 biochemistry & molecular biology
biology
Indoleacetic Acids
Arabidopsis Proteins
fungi
Autophagosomes
food and beverages
Cell Biology
Meristem maintenance
biology.organism_classification
Ascorbic acid
Cell biology
030104 developmental biology
Glucose
chemistry
root meristem
biology.protein
Signal Transduction
Research Paper
Subjects
Details
- ISSN :
- 15548635
- Volume :
- 15
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Autophagy
- Accession number :
- edsair.doi.dedup.....13063207a090e7832779d3daf43b9d91