Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.Predlozhen uproshchennyĭ sposob vydeleniia ékzosom iz plazmy krovi, pozvoliaiushchiĭ poluchit' vysokoochishchennye preparaty mikrovezikul razmerom ne bolee 100 nm. Ustanovleno nalichie razlichnykh subpopuliatsiĭ ékzosom kak v plazme krovi zdorovykh donorov, tak i onkologicheskikh bol'nykh. Universal'nye markery CD9, CD24 i CD81 mogut byt' ispol'zovany dlia rutinnogo tipirovaniia ékzosom.