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Breast milk-derived exosomes promote intestinal epithelial cell growth
- Source :
- Journal of Pediatric Surgery. 52:755-759
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Background Breast milk administration prevents necrotizing enterocolitis (NEC). However, the mechanism remains unclear. Exosomes are cell-derived vesicles highly present in human milk and regulate intercellular signaling, inflammation, and immune response. We hypothesized that milk-derived exosomes beneficially affect intestinal epithelial cells. Methods Rat milk was collected, and exosomes were isolated using ExoQuick reagent and visualized by Nanoparticle Tracking Analysis. Protein was extracted from encapsulating exosomes, and concentration was measured. 2×10 4 intestinal epithelial cells (IEC-18) were treated for five hours with 0.5-μg/μl exosomes, an equal volume of exosome-free milk, or control solution (PBS). IEC-18 viability was measured using a colorimetric assay (MTT), and gene expression was analyzed by qRT-PCR. Data were compared using one-way ANOVA with Bonferroni post-test. Results Rat milk was collected, and exosome isolation was confirmed. Compared to control, treatment with exosomes significantly increased IEC viability, proliferation, and stem cell activity (all p Conclusions Rat milk-derived exosomes promote IEC viability, enhance proliferation, and stimulate intestinal stem cell activity. These findings provide insight into the mechanism of action of breast milk in the intestines. Exosome administration is a promising prevention method for infants at risk of developing NEC when breastfeeding is not tolerated.
- Subjects :
- 0301 basic medicine
Cell viability
Breast milk
Cell Survival
Proliferation
Inflammation
Biology
Exosomes
Pediatrics
Exosome
Andrology
03 medical and health sciences
Immune system
Enterocolitis, Necrotizing
medicine
Animals
Humans
Viability assay
Intestinal Mucosa
Cell Proliferation
Enterocolitis
Infant, Newborn
Infant
Epithelial Cells
General Medicine
Perinatology and Child Health
Newborn
Microvesicles
Epithelium
Rats
Necrotizing enterocolitis (NEC)
Breast Feeding
Milk
030104 developmental biology
medicine.anatomical_structure
Pediatrics, Perinatology and Child Health
Immunology
Female
Surgery
medicine.symptom
Stem cell
Necrotizing
Subjects
Details
- ISSN :
- 00223468
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Journal of Pediatric Surgery
- Accession number :
- edsair.doi.dedup.....12ccd543e6f64f112689287ab4a913df