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Development and application of a quadruplex realātime PCR assay for differential detection of porcine circoviruses (PCV1 to PCV4) in Jiangsu province of China from 2016 to 2020
- Source :
- Transboundary and Emerging Diseases. 68:1615-1624
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- To date, four species of porcine circoviruses (PCVs), including PCV1-4, have been reported to exist in the clinical cases. Fast and effective differential detection is critical to monitor the infection and co-infection status of PCVs for adopting reliable control strategies. However, currently available methods cannot simultaneously differentiate the four species of PCV strains. In this study, a quadruplex real-time PCR assay based on TaqMan probes was developed for differential detection of PCV1-4. The new quadruplex real-time PCR assay exhibited satisfied specificity, sensitivity, repeatability and reproducibility. In addition, the new assay was applied to the detection of 120 clinical samples collected from 2016 to 2020 in Jiangsu province of China and compared with previously reported PCV1-4 singleplex conventional PCR assays. Based on the clinical performance, the results from the quadruplex real-time PCR and conventional PCR assays showed 100% agreement. A total of 47 samples were detected as PCV positive by the quadruplex real-time PCR assay, including 1, 2, 1 samples were co-infected with PCV1 and PCV4, PCV2 and PCV3, PCV2 and PCV4, respectively. Full-length ORF2 sequencing and phylogenetic analysis supported the real-time PCR results that 5, 34, 8 and 4 of the 51 PCV sequences were PCV1, PCV2, PCV3 and PCV4, respectively. This study provides a promising alternative tool for rapid differential detection of PCVs and confirms the coexistence of all species of PCV1-4 strains in Jiangsu province in recent years.
- Subjects :
- Circovirus
China
Swine
040301 veterinary sciences
Sus scrofa
Pcr assay
Biology
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
0403 veterinary science
Porcine Circoviruses
03 medical and health sciences
Prevalence
TaqMan
Animals
Circoviridae Infections
030304 developmental biology
Swine Diseases
0303 health sciences
General Veterinary
General Immunology and Microbiology
Clinical performance
Reproducibility of Results
04 agricultural and veterinary sciences
General Medicine
Virology
Real-time polymerase chain reaction
Subjects
Details
- ISSN :
- 18651682 and 18651674
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Transboundary and Emerging Diseases
- Accession number :
- edsair.doi.dedup.....12bcfa3d6d944e291d6355e6c833d0ce