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3-O-methylfunicone produced by penicillium pinophilum affects cell motility of breast cancer cells, downregulating alphavbeta5 integrin and inhibiting metalloproteinase-9 secretion
- Source :
- Molecular carcinogenesis, 46 (2007): 930–940. doi:10.1002/mc.20322, info:cnr-pdr/source/autori:E. Buommino, M. Boccellino, A. De Filippis, M. Petrazzuolo, V. Cozza, R. Nicoletti, M. L. Ciavatta, L. Quagliuolo, M. A. Tufano./titolo:3-O-methylfunicone produced by Penicillium pinophilum affects cell motilità of breast cancer cells, downregulating avb5 integrin and inhibiting metalloproteinase-9 secretion./doi:10.1002%2Fmc.20322/rivista:Molecular carcinogenesis (Print)/anno:2007/pagina_da:930/pagina_a:940/intervallo_pagine:930–940/volume:46
- Publication Year :
- 2007
-
Abstract
- Recent evidence assigns integrins and metalloproteinases (MMPs) an important role in regulating tumor cell progression. Here, we demonstrate that 3-O-methylfunicone (OMF), a secondary metabolite produced by Penicillium pinophilum, affects cell proliferation and motility of breast cancer MCF-7 cells, downregulating avb5 integrin, and inhibiting MMP-9 secretion. This effect was absent when the non-tumoral MCF-10 cell line was used. Inhibition of cell motility was also associated to modifications in cell shape and in the distribution of tubulin fibers of OMF-treated MCF- 7 cells. In addition, a possible effect on survivin and hTERT was also investigated. We found that OMF strongly inhibits survivin and hTERT gene expression. The results of this study indicate that OMF-induced inhibition of cell motility may be mediated through the modulation of avb5 integrin and MMP-9secretion. In addition, the inhibition of typical markers of tumor progression such as hTERT and survivin in MCF-7 and their inactivity towards MCF10 provide strong evidence for a potential use of OMF in anticancer therapy Recent evidence assigns integrins and metalloproteinases (MMPs) an important role in regulating tumor cell progression. Here, we demonstrate that 3-O-methylfunicone (OMF), a secondary metabolite produced by Penicillium pinophilum, affects cell proliferation and motility of breast cancer MCF-7 cells, downregulating αvβ5 integrin, and inhibiting MMP-9 secretion. This effect was absent when the non-tumoral MCF-10 cell line was used. Inhibition of cell motility was also associated to modifications in cell shape and in the distribution of tubulin fibers of OMF-treated MCF-7 cells. In addition, a possible effect on survivin and hTERT was also investigated. We found that OMF strongly inhibits survivin and hTERT gene expression. The results of this study indicate that OMF-induced inhibition of cell motility may be mediated through the modulation of αvβ5 integrin and MMP-9 secretion. In addition, the inhibition of typical markers of tumor progression such as hTERT and survivin in MCF-7 and their inactivity towards MCF10 provide strong evidence for a potential use of OMF in anticancer therapy. © 2007 Wiley-Liss, Inc.
- Subjects :
- Cancer Research
Integrins
Integrin
Motility
Down-Regulation
Breast Neoplasms
Matrix metalloproteinase
cell motility
metalloproteinases
Cell Movement
Tubulin
Cell Line, Tumor
Survivin
Humans
Secretion
Receptors, Vitronectin
OMF
Molecular Biology
Cell Shape
Metalloproteinase
Cell Proliferation
OMF,cell motility,integrins,metalloproteinases
Fibrin
biology
Cell growth
Penicillium
Cell biology
Matrix Metalloproteinase 9
Cell culture
Tumor progression
Pyrones
biology.protein
Drug Evaluation
Female
Subjects
Details
- ISSN :
- 08991987
- Volume :
- 46
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Molecular carcinogenesis
- Accession number :
- edsair.doi.dedup.....12b4feae7353db21dd0f9239c0a870ea